WEKO3
-
RootNode
アイテム
There is a newer version of this record available.
Characterization of lectin isolated from Momordica charantia seed as a B cell activator
http://hdl.handle.net/10458/2876
http://hdl.handle.net/10458/2876e8368b88-d45e-4a5d-8c55-fd2e8d522536
名前 / ファイル | ライセンス | アクション |
---|---|---|
![]() |
|
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2010-05-26 | |||||
タイトル | ||||||
タイトル | Characterization of lectin isolated from Momordica charantia seed as a B cell activator | |||||
言語 | en | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
キーワード | Momordica charantia seed lectin, B cell activator, T cell independent, BALB/c mice | |||||
資源タイプ | ||||||
資源タイプ | journal article | |||||
著者 |
Huang, Li
× Huang, Li× Adachi, Takumi× Shimizu, Yuya× Goto, Yoshitaka× Toyama, Jun× Tanaka, Hidenori× Akashi, Ryo× Sawaguchi, Akira× Iwata, Hiroyuki× Haga, Takeshi |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Lectin isolated from the seeds of Momordica charantia (MCL) is a galactose-specific glycoprotein. To investigate the effects of MCL on cell activation, we analyzed the responses of BALB/c splenocytes, thymocytes, T cells and B cells on MCL stimulation. Proliferation assays showed that MCL selectively stimulates the B cell subset of splenocytes (p < 0.05) in a dose and time dependent manner and that this activation proceeds without the involvement of T cells. Flow cytometric analysis revealed that the fluorescein isothiocyanate (FITC)-labeled MCL binds to B cells, which was inhibited by specific sugars, including galactose. Mouse immunoglobulin (Ig) was able to inhibit MCL-induced proliferation of mouse B cells, suggesting MCL stimulates B cell activation via membrane Ig in the B cell surface. Moreover, after 96-h co-culture, MCL triggered splenocytes to produce a large amount of non-specific IgM in culture supernatants (p < 0.01). Additionally, MCL was shown to up-regulate the cell activation marker CD86, in a B cell subpopulation distinct from that affected by LPS. These data suggest that MCL is a T cell-independent B cell activator and a polyclonal Ig inducer, and provide further information on the immunomodulatory effect of MCL. | |||||
言語 | en | |||||
書誌情報 |
en : Immunology letters 巻 121, 号 2, p. 148-156, 発行日 2008-12-22 |
|||||
出版者 | ||||||
出版者 | Elsevier Science | |||||
言語 | en | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 01652478 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00231960 | |||||
PubMed番号 | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | PMID | |||||
関連識別子 | 19014972 | |||||
著者版フラグ | ||||||
出版タイプ | AM |
Share
Cite as
Huang, Li, Adachi, Takumi, Shimizu, Yuya, Goto, Yoshitaka, Toyama, Jun, Tanaka, Hidenori, Akashi, Ryo, Sawaguchi, Akira, Iwata, Hiroyuki, Haga, Takeshi, 2008, Characterization of lectin isolated from Momordica charantia seed as a B cell activator: Elsevier Science, 148–156 p.
Loading...