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Characterization of lectin isolated from Momordica charantia seed as a B cell activator
http://hdl.handle.net/10458/2876
http://hdl.handle.net/10458/28760102b47e-2633-4aa7-be67-72c1b42d5e95
| 名前 / ファイル | ライセンス | アクション |
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2876.pdf (631.0 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||||||
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| 公開日 | 2010-05-26 | |||||||||||||||||
| タイトル | ||||||||||||||||||
| タイトル | Characterization of lectin isolated from Momordica charantia seed as a B cell activator | |||||||||||||||||
| 言語 | en | |||||||||||||||||
| 言語 | ||||||||||||||||||
| 言語 | eng | |||||||||||||||||
| キーワード | ||||||||||||||||||
| 言語 | en | |||||||||||||||||
| キーワード | Momordica charantia seed lectin, B cell activator, T cell independent, BALB/c mice | |||||||||||||||||
| 資源タイプ | ||||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||
| 著者 |
Huang, Li
× Huang, Li× Adachi, Takumi× Shimizu, Yuya× Goto, Yoshitaka× Toyama, Jun× 田中, 秀典
WEKO
4740
× 明石, 良
WEKO
1585
× Sawaguchi, Akira× Iwata, Hiroyuki× Haga, Takeshi |
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| 抄録 | ||||||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||||||
| 内容記述 | Lectin isolated from the seeds of Momordica charantia （MCL） is a galactose-specific glycoprotein. To investigate the effects of MCL on cell activation, we analyzed the responses of BALB/c splenocytes, thymocytes, T cells and B cells on MCL stimulation. Proliferation assays showed that MCL selectively stimulates the B cell subset of splenocytes （p < 0.05） in a dose and time dependent manner and that this activation proceeds without the involvement of T cells. Flow cytometric analysis revealed that the fluorescein isothiocyanate （FITC）-labeled MCL binds to B cells, which was inhibited by specific sugars, including galactose. Mouse immunoglobulin （Ig） was able to inhibit MCL-induced proliferation of mouse B cells, suggesting MCL stimulates B cell activation via membrane Ig in the B cell surface. Moreover, after 96-h co-culture, MCL triggered splenocytes to produce a large amount of non-specific IgM in culture supernatants （p < 0.01）. Additionally, MCL was shown to up-regulate the cell activation marker CD86, in a B cell subpopulation distinct from that affected by LPS. These data suggest that MCL is a T cell-independent B cell activator and a polyclonal Ig inducer, and provide further information on the immunomodulatory effect of MCL. | |||||||||||||||||
| 言語 | en | |||||||||||||||||
| 書誌情報 |
en : Immunology letters 巻 121, 号 2, p. 148-156, 発行日 2008-12-22 |
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| 出版者 | Elsevier Science | |||||||||||||||||
| 言語 | en | |||||||||||||||||
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| 収録物識別子タイプ | ISSN | |||||||||||||||||
| 収録物識別子 | 01652478 | |||||||||||||||||
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| 収録物識別子タイプ | NCID | |||||||||||||||||
| 収録物識別子 | AA00231960 | |||||||||||||||||
| PubMed番号 | ||||||||||||||||||
| 関連タイプ | isVersionOf | |||||||||||||||||
| 識別子タイプ | PMID | |||||||||||||||||
| 関連識別子 | 19014972 | |||||||||||||||||
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| 出版タイプ | AM | |||||||||||||||||
