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Characterization of lectin isolated from Momordica charantia seed as a B cell activator
http://hdl.handle.net/10458/2876
http://hdl.handle.net/10458/28769602a61a-8a63-4019-87fc-7ee45284609c
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
2876.pdf (631.0 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2010-05-26 | |||||
| タイトル | ||||||
| タイトル | Characterization of lectin isolated from Momordica charantia seed as a B cell activator | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | Momordica charantia seed lectin, B cell activator, T cell independent, BALB/c mice | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| 著者 |
Huang, Li
× Huang, Li× Adachi, Takumi× Shimizu, Yuya× Goto, Yoshitaka× Toyama, Jun× Tanaka, Hidenori× Akashi, Ryo× Sawaguchi, Akira× Iwata, Hiroyuki× Haga, Takeshi |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Lectin isolated from the seeds of Momordica charantia （MCL） is a galactose-specific glycoprotein. To investigate the effects of MCL on cell activation, we analyzed the responses of BALB/c splenocytes, thymocytes, T cells and B cells on MCL stimulation. Proliferation assays showed that MCL selectively stimulates the B cell subset of splenocytes （p < 0.05） in a dose and time dependent manner and that this activation proceeds without the involvement of T cells. Flow cytometric analysis revealed that the fluorescein isothiocyanate （FITC）-labeled MCL binds to B cells, which was inhibited by specific sugars, including galactose. Mouse immunoglobulin （Ig） was able to inhibit MCL-induced proliferation of mouse B cells, suggesting MCL stimulates B cell activation via membrane Ig in the B cell surface. Moreover, after 96-h co-culture, MCL triggered splenocytes to produce a large amount of non-specific IgM in culture supernatants （p < 0.01）. Additionally, MCL was shown to up-regulate the cell activation marker CD86, in a B cell subpopulation distinct from that affected by LPS. These data suggest that MCL is a T cell-independent B cell activator and a polyclonal Ig inducer, and provide further information on the immunomodulatory effect of MCL. | |||||
| 言語 | en | |||||
| 書誌情報 |
en : Immunology letters 巻 121, 号 2, p. 148-156, 発行日 2008-12-22 |
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| 出版者 | ||||||
| 出版者 | Elsevier Science | |||||
| 言語 | en | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 01652478 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA00231960 | |||||
| PubMed番号 | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 19014972 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
