{"created":"2023-07-29T12:55:00.951018+00:00","links":{},"metadata":{"_buckets":{"deposit":"db203ce7-6dab-483b-9f3b-90c15a9a09e2"},"_deposit":{"created_by":9,"id":"765.2","owner":"5","owners":[5],"pid":{"revision_id":0,"type":"depid","value":"765.2"},"status":"published"},"_oai":{"id":"oai:miyazaki-u.repo.nii.ac.jp:00000765.2","sets":["74","74:26"]},"author_link":["4650","4651","4614","2693","4654","4740","1585","4657","4658","2692"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-12-22","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"156","bibliographicPageStart":"148","bibliographicVolumeNumber":"121","bibliographic_titles":[{"bibliographic_title":"Immunology letters","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Lectin isolated from the seeds of Momordica charantia （MCL） is a galactose-specific glycoprotein. To investigate the effects of MCL on cell activation, we analyzed the responses of BALB/c splenocytes, thymocytes, T cells and B cells on MCL stimulation. Proliferation assays showed that MCL selectively stimulates the B cell subset of splenocytes （p < 0.05） in a dose and time dependent manner and that this activation proceeds without the involvement of T cells. Flow cytometric analysis revealed that the fluorescein isothiocyanate （FITC）-labeled MCL binds to B cells, which was inhibited by specific sugars, including galactose. Mouse immunoglobulin （Ig） was able to inhibit MCL-induced proliferation of mouse B cells, suggesting MCL stimulates B cell activation via membrane Ig in the B cell surface. Moreover, after 96-h co-culture, MCL triggered splenocytes to produce a large amount of non-specific IgM in culture supernatants （p < 0.01）. Additionally, MCL was shown to up-regulate the cell activation marker CD86, in a B cell subpopulation distinct from that affected by LPS. These data suggest that MCL is a T cell-independent B cell activator and a polyclonal Ig inducer, and provide further information on the immunomodulatory effect of MCL.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier Science","subitem_publisher_language":"en"}]},"item_10001_relation_13":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"19014972","subitem_relation_type_select":"PMID"}}]},"item_10001_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00231960","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"01652478","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Huang, Li","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4650","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Adachi, Takumi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4651","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Shimizu, Yuya","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4614","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Goto, Yoshitaka","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"2693","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Toyama, Jun","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4654","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tanaka, Hidenori","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4740","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"50529253","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=50529253"}]},{"creatorNames":[{"creatorName":"Akashi, Ryo","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1585","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"20253809","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=20253809"}]},{"creatorNames":[{"creatorName":"Sawaguchi, Akira","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4657","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Iwata, Hiroyuki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4658","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Haga, Takeshi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"2692","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-06-21"}],"displaytype":"detail","filename":"2876.pdf","filesize":[{"value":"631.0 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"2876.pdf","url":"https://miyazaki-u.repo.nii.ac.jp/record/765/files/2876.pdf"},"version_id":"2acea785-4e70-4a00-99b3-555684cfa036"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Momordica charantia seed lectin, B cell activator, T cell independent, BALB/c mice","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Characterization of lectin isolated from Momordica charantia seed as a B cell activator","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Characterization of lectin isolated from Momordica charantia seed as a B cell activator","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"5","path":["74","26"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2010-05-26"},"publish_date":"2010-05-26","publish_status":"0","recid":"765.2","relation_version_is_last":true,"title":["Characterization of lectin isolated from Momordica charantia seed as a B cell activator"],"weko_creator_id":"5","weko_shared_id":2},"updated":"2023-07-29T12:55:04.544603+00:00"}