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REGULATION OF FOWL SPERM FLAGELLAR MOTILITY BY PROTEIN PHOSPHATASE TYPE 1 AND ITS RELATIONSHIP WITH DEPHOSPHORYLATION OF AXONEMAL AND/OR ACCESSORY CYTOSKELETAL PROTEINS
http://hdl.handle.net/10458/716
http://hdl.handle.net/10458/716d93e98ce-08c4-41b5-ba79-4963dba72e96
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
ashizawa60.pdf (884.4 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2007-10-10 | |||||
| タイトル | ||||||
| タイトル | REGULATION OF FOWL SPERM FLAGELLAR MOTILITY BY PROTEIN PHOSPHATASE TYPE 1 AND ITS RELATIONSHIP WITH DEPHOSPHORYLATION OF AXONEMAL AND/OR ACCESSORY CYTOSKELETAL PROTEINS | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | ja | |||||
| 主題Scheme | Other | |||||
| キーワード | 精子 | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | sperm | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| 著者 |
芦沢, 幸二
× 芦沢, 幸二× Hashimoto, Kei× 續木, 靖浩 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The motility of demembranated fowl spermatozoa was vigorous at 30°C in the presence of ATP, but decreased markedly following the addition of recombinant protein phosphatase type 1 （PP-1） supplemented with Mn2+. This inhibition was not restored by the addition of cAMP, within the range 1-1000 μM, but instantly restored by the addition of 50 ng/ml trypsin. Phosphorylation of demembranated fowl sperm proteins during incubation with [γ-32P]ATP at 30°C was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis （SDS-PAGE）. A marked difference in phosphorylation status was observed in approximately 116, 86, 79, 50 and 29-kDa proteins. These proteins were dephosphorylated in the presence of PP-1 and Mn2+ compared with those in control samples. These results suggest that PP-1-mediated dephosphorylation of some of these proteins of the axoneme and/or accessory cytoskeletal components of fowl spermatozoa may be involved in the inhibition of motility. | |||||
| 言語 | en | |||||
| 書誌情報 |
en : Biochemical and biophysical research communications 巻 235, 号 1, p. 108-112, 発行日 1997 |
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| 出版者 | ||||||
| 出版者 | Elsevier | |||||
| 言語 | en | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0006291X | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA00564395 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
