@article{oai:miyazaki-u.repo.nii.ac.jp:00000263,
 author = {Ashizawa, Koji and 芦沢, 幸二 and Hashimoto, Kei and Tsuzuki, Yasuhiro and 續木, 靖浩},
 issue = {1},
 journal = {Biochemical and biophysical research communications},
 month = {},
 note = {The motility of demembranated fowl spermatozoa was vigorous at 30°C in the presence of ATP, but decreased markedly following the addition of recombinant protein phosphatase type 1 （PP-1） supplemented with Mn2+. This inhibition was not restored by the addition of cAMP, within the range 1-1000 μM, but instantly restored by the addition of 50 ng/ml trypsin. Phosphorylation of demembranated fowl sperm proteins during incubation with [γ-32P]ATP at 30°C was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis （SDS-PAGE）. A marked difference in phosphorylation status was observed in approximately 116, 86, 79, 50 and 29-kDa proteins. These proteins were dephosphorylated in the presence of PP-1 and Mn2+ compared with those in control samples. These results suggest that PP-1-mediated dephosphorylation of some of these proteins of the axoneme and/or accessory cytoskeletal components of fowl spermatozoa may be involved in the inhibition of motility.},
 pages = {108--112},
 title = {REGULATION OF FOWL SPERM FLAGELLAR MOTILITY BY PROTEIN PHOSPHATASE TYPE 1 AND ITS RELATIONSHIP WITH DEPHOSPHORYLATION OF AXONEMAL AND/OR ACCESSORY CYTOSKELETAL PROTEINS},
 volume = {235},
 year = {1997},
 yomi = {アシザワ, コウジ and ツヅキ, ヤスヒロ}
}