{"created":"2023-05-15T09:56:37.977820+00:00","id":263,"links":{},"metadata":{"_buckets":{"deposit":"1e53fc3e-fcd4-4d68-bf28-b8972f24897a"},"_deposit":{"created_by":5,"id":"263","owner":"5","owners":[5],"pid":{"revision_id":0,"type":"depid","value":"263"},"status":"published"},"_oai":{"id":"oai:miyazaki-u.repo.nii.ac.jp:00000263","sets":["74","74:26"]},"author_link":["1043","2104","932"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1997","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"112","bibliographicPageStart":"108","bibliographicVolumeNumber":"235","bibliographic_titles":[{"bibliographic_title":"Biochemical and biophysical research communications","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The motility of demembranated fowl spermatozoa was vigorous at 30°C in the presence of ATP, but decreased markedly following the addition of recombinant protein phosphatase type 1 （PP-1） supplemented with Mn2+. This inhibition was not restored by the addition of cAMP, within the range 1-1000 μM, but instantly restored by the addition of 50 ng/ml trypsin. Phosphorylation of demembranated fowl sperm proteins during incubation with [γ-32P]ATP at 30°C was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis （SDS-PAGE）. A marked difference in phosphorylation status was observed in approximately 116, 86, 79, 50 and 29-kDa proteins. These proteins were dephosphorylated in the presence of PP-1 and Mn2+ compared with those in control samples. These results suggest that PP-1-mediated dephosphorylation of some of these proteins of the axoneme and/or accessory cytoskeletal components of fowl spermatozoa may be involved in the inhibition of motility.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier","subitem_publisher_language":"en"}]},"item_10001_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00564395","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0006291X","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"Ashizawa, Koji","creatorNameLang":"en"},{"creatorName":"芦沢, 幸二","creatorNameLang":"ja"},{"creatorName":"アシザワ, コウジ","creatorNameLang":"ja-Kana"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hashimoto, Kei","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"Tsuzuki, Yasuhiro","creatorNameLang":"en"},{"creatorName":"續木, 靖浩","creatorNameLang":"ja"},{"creatorName":"ツヅキ, ヤスヒロ","creatorNameLang":"ja-Kana"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-06-21"}],"displaytype":"detail","filename":"ashizawa60.pdf","filesize":[{"value":"884.4 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"ashizawa60.pdf","url":"https://miyazaki-u.repo.nii.ac.jp/record/263/files/ashizawa60.pdf"},"version_id":"2aa46a0b-2596-411c-ba4c-907a00a9744e"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"精子","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"sperm","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"REGULATION OF FOWL SPERM FLAGELLAR MOTILITY BY PROTEIN PHOSPHATASE TYPE 1 AND ITS RELATIONSHIP WITH DEPHOSPHORYLATION OF AXONEMAL AND/OR ACCESSORY CYTOSKELETAL PROTEINS","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"REGULATION OF FOWL SPERM FLAGELLAR MOTILITY BY PROTEIN PHOSPHATASE TYPE 1 AND ITS RELATIONSHIP WITH DEPHOSPHORYLATION OF AXONEMAL AND/OR ACCESSORY CYTOSKELETAL PROTEINS","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"5","path":["74","26"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2007-10-10"},"publish_date":"2007-10-10","publish_status":"0","recid":"263","relation_version_is_last":true,"title":["REGULATION OF FOWL SPERM FLAGELLAR MOTILITY BY PROTEIN PHOSPHATASE TYPE 1 AND ITS RELATIONSHIP WITH DEPHOSPHORYLATION OF AXONEMAL AND/OR ACCESSORY CYTOSKELETAL PROTEINS"],"weko_creator_id":"5","weko_shared_id":-1},"updated":"2023-10-09T05:34:24.182929+00:00"}