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REGULATION OF FOWL SPERM FLAGELLAR MOTILITY BY PROTEIN PHOSPHATASE TYPE 1 AND ITS RELATIONSHIP WITH DEPHOSPHORYLATION OF AXONEMAL AND/OR ACCESSORY CYTOSKELETAL PROTEINS
http://hdl.handle.net/10458/716
http://hdl.handle.net/10458/7168e7d550e-e2c4-4882-b0a9-792e1e8f6fc2
名前 / ファイル | ライセンス | アクション |
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ashizawa60.pdf (884.4 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2007-10-10 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | REGULATION OF FOWL SPERM FLAGELLAR MOTILITY BY PROTEIN PHOSPHATASE TYPE 1 AND ITS RELATIONSHIP WITH DEPHOSPHORYLATION OF AXONEMAL AND/OR ACCESSORY CYTOSKELETAL PROTEINS | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
言語 | ja | |||||
主題Scheme | Other | |||||
キーワード | 精子 | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
キーワード | sperm | |||||
資源タイプ | ||||||
資源タイプ | journal article | |||||
著者 |
芦沢, 幸二
× 芦沢, 幸二× Hashimoto, Kei× Tsuzuki, Yasuhiro |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The motility of demembranated fowl spermatozoa was vigorous at 30°C in the presence of ATP, but decreased markedly following the addition of recombinant protein phosphatase type 1 (PP-1) supplemented with Mn2+. This inhibition was not restored by the addition of cAMP, within the range 1-1000 μM, but instantly restored by the addition of 50 ng/ml trypsin. Phosphorylation of demembranated fowl sperm proteins during incubation with [γ-32P]ATP at 30°C was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). A marked difference in phosphorylation status was observed in approximately 116, 86, 79, 50 and 29-kDa proteins. These proteins were dephosphorylated in the presence of PP-1 and Mn2+ compared with those in control samples. These results suggest that PP-1-mediated dephosphorylation of some of these proteins of the axoneme and/or accessory cytoskeletal components of fowl spermatozoa may be involved in the inhibition of motility. | |||||
言語 | en | |||||
書誌情報 |
en : Biochemical and biophysical research communications 巻 235, 号 1, p. 108-112, 発行日 1997 |
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出版者 | ||||||
言語 | en | |||||
出版者 | Elsevier | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0006291X | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00564395 | |||||
著者版フラグ | ||||||
出版タイプ | AM |