{"created":"2023-07-29T13:20:26.227680+00:00","links":{},"metadata":{"_buckets":{"deposit":"900bc9c4-fe7d-4b44-b73f-677a17a373e7"},"_deposit":{"created_by":9,"id":"263.2","owner":"5","owners":[5],"pid":{"revision_id":0,"type":"depid","value":"263.2"},"status":"published"},"_oai":{"id":"oai:miyazaki-u.repo.nii.ac.jp:00000263.2","sets":["74","74:26"]},"author_link":["932","1043","2104"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1997","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"112","bibliographicPageStart":"108","bibliographicVolumeNumber":"235","bibliographic_titles":[{"bibliographic_title":"Biochemical and biophysical research communications","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The motility of demembranated fowl spermatozoa was vigorous at 30°C in the presence of ATP, but decreased markedly following the addition of recombinant protein phosphatase type 1 （PP-1） supplemented with Mn2+. This inhibition was not restored by the addition of cAMP, within the range 1-1000 μM, but instantly restored by the addition of 50 ng/ml trypsin. Phosphorylation of demembranated fowl sperm proteins during incubation with [γ-32P]ATP at 30°C was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis （SDS-PAGE）. A marked difference in phosphorylation status was observed in approximately 116, 86, 79, 50 and 29-kDa proteins. These proteins were dephosphorylated in the presence of PP-1 and Mn2+ compared with those in control samples. These results suggest that PP-1-mediated dephosphorylation of some of these proteins of the axoneme and/or accessory cytoskeletal components of fowl spermatozoa may be involved in the inhibition of motility.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier","subitem_publisher_language":"en"}]},"item_10001_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00564395","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0006291X","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"芦沢, 幸二","creatorNameLang":"ja"},{"creatorName":"Ashizawa, Koji","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hashimoto, Kei","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tsuzuki, Yasuhiro","creatorNameLang":"en"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-06-21"}],"displaytype":"detail","filename":"ashizawa60.pdf","filesize":[{"value":"884.4 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"ashizawa60.pdf","url":"https://miyazaki-u.repo.nii.ac.jp/record/263/files/ashizawa60.pdf"},"version_id":"43bd2160-be4d-4aeb-aaaa-b45b0324739c"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"精子","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"sperm","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"REGULATION OF FOWL SPERM FLAGELLAR MOTILITY BY PROTEIN PHOSPHATASE TYPE 1 AND ITS RELATIONSHIP WITH DEPHOSPHORYLATION OF AXONEMAL AND/OR ACCESSORY CYTOSKELETAL PROTEINS","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"REGULATION OF FOWL SPERM FLAGELLAR MOTILITY BY PROTEIN PHOSPHATASE TYPE 1 AND ITS RELATIONSHIP WITH DEPHOSPHORYLATION OF AXONEMAL AND/OR ACCESSORY CYTOSKELETAL PROTEINS","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"5","path":["74","26"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2007-10-10"},"publish_date":"2007-10-10","publish_status":"0","recid":"263.2","relation_version_is_last":true,"title":["REGULATION OF FOWL SPERM FLAGELLAR MOTILITY BY PROTEIN PHOSPHATASE TYPE 1 AND ITS RELATIONSHIP WITH DEPHOSPHORYLATION OF AXONEMAL AND/OR ACCESSORY CYTOSKELETAL PROTEINS"],"weko_creator_id":"5","weko_shared_id":-1},"updated":"2023-07-29T13:20:29.304334+00:00"}