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In vitro Effects of β-Carotene for the Motility, ATP and Intracellular Free Ca2+ Concentrations of Fowl Spermatozoa
http://hdl.handle.net/10458/735
http://hdl.handle.net/10458/73529ec9cdd-11f5-452b-873e-0223bcf6921d
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
ashizawa73.pdf (1.3 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2007-10-11 | |||||
| タイトル | ||||||
| タイトル | In vitro Effects of β-Carotene for the Motility, ATP and Intracellular Free Ca2+ Concentrations of Fowl Spermatozoa | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | ja | |||||
| 主題Scheme | Other | |||||
| キーワード | 精子 | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | sperm, Spermatozoa, β-carotene, Motility, ATP concentrations, Intracellular free calcium concentrations | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| その他（別言語等）のタイトル | ||||||
| その他のタイトル | β-carotene and fowl sperm function | |||||
| 言語 | en | |||||
| 著者 |
芦沢, 幸二
× 芦沢, 幸二× Tomita, Yoshifumi× Kanemaru, Chikako× 續木, 靖浩 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The motility of intact fowl spermatozoa was vigorous at 25°C, but decreased gradually following the addition of 0-100 μM β-carotene in a dose-dependent manner. Even in the presence of stimulators of fowl sperm motility, such as Ca2+ or calyculin A, the motility of intact spermatozoa at both 25°C and 40°C remained inhibited following the addition of β- carotene. Under all of these circumstances, sperm ATP concentrations were not reduced by the addition of β-carotene. Moreover, the motility of demembranated spermatozoa was not inhibited by the addition of the same concentrations of β-carotene. No changes in intracellular free Ca2+ concentrations, measured by means of a fluorescent Ca2+ indicator, fura-2, were observed in intact β-carotene -treated spermatozoa. These results suggest that β-carotene is involved in the inhibition of the flagellar movement of fowl spermatozoa without change in energy production, and that the target of β-carotene might be present in the cytoplasmic matrix and/or the plasma membrane, but not retained in the axoneme and/or accessory cytoskeletal components. | |||||
| 言語 | en | |||||
| 書誌情報 |
en : Molecular reproduction and development 巻 56, 号 1, p. 99-104, 発行日 2000 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1040452X | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA10735246 | |||||
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| 出版タイプ | AM | |||||
