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In vitro Effects of β-Carotene for the Motility, ATP and Intracellular Free Ca2+ Concentrations of Fowl Spermatozoa
http://hdl.handle.net/10458/735
http://hdl.handle.net/10458/73529ec9cdd-11f5-452b-873e-0223bcf6921d
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
ashizawa73.pdf (1.3 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||
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| 公開日 | 2007-10-11 | |||||||||||
| タイトル | ||||||||||||
| タイトル | In vitro Effects of β-Carotene for the Motility, ATP and Intracellular Free Ca2+ Concentrations of Fowl Spermatozoa | |||||||||||
| 言語 | en | |||||||||||
| 言語 | ||||||||||||
| 言語 | eng | |||||||||||
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| 言語 | ja | |||||||||||
| キーワード | 精子 | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| キーワード | sperm, Spermatozoa, β-carotene, Motility, ATP concentrations, Intracellular free calcium concentrations | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ | journal article | |||||||||||
| その他（別言語等）のタイトル | ||||||||||||
| その他のタイトル | β-carotene and fowl sperm function | |||||||||||
| 言語 | en | |||||||||||
| 著者 |
芦沢, 幸二
× 芦沢, 幸二× Tomita, Yoshifumi× Kanemaru, Chikako× 續木, 靖浩
WEKO
2104
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| 抄録 | ||||||||||||
| 内容記述タイプ | Abstract | |||||||||||
| 内容記述 | The motility of intact fowl spermatozoa was vigorous at 25°C, but decreased gradually following the addition of 0-100 μM β-carotene in a dose-dependent manner. Even in the presence of stimulators of fowl sperm motility, such as Ca2+ or calyculin A, the motility of intact spermatozoa at both 25°C and 40°C remained inhibited following the addition of β- carotene. Under all of these circumstances, sperm ATP concentrations were not reduced by the addition of β-carotene. Moreover, the motility of demembranated spermatozoa was not inhibited by the addition of the same concentrations of β-carotene. No changes in intracellular free Ca2+ concentrations, measured by means of a fluorescent Ca2+ indicator, fura-2, were observed in intact β-carotene -treated spermatozoa. These results suggest that β-carotene is involved in the inhibition of the flagellar movement of fowl spermatozoa without change in energy production, and that the target of β-carotene might be present in the cytoplasmic matrix and/or the plasma membrane, but not retained in the axoneme and/or accessory cytoskeletal components. | |||||||||||
| 言語 | en | |||||||||||
| 書誌情報 |
en : Molecular reproduction and development 巻 56, 号 1, p. 99-104, 発行日 2000 |
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| 収録物識別子タイプ | ISSN | |||||||||||
| 収録物識別子 | 1040452X | |||||||||||
| 書誌レコードID | ||||||||||||
| 収録物識別子タイプ | NCID | |||||||||||
| 収録物識別子 | AA10735246 | |||||||||||
| 著者版フラグ | ||||||||||||
| 出版タイプ | AM | |||||||||||
