{"created":"2023-05-15T09:57:05.478470+00:00","id":849,"links":{},"metadata":{"_buckets":{"deposit":"3a0ed4c5-356e-4c5e-9e91-6ec53eb803ec"},"_deposit":{"created_by":5,"id":"849","owner":"5","owners":[5],"pid":{"revision_id":0,"type":"depid","value":"849"},"status":"published"},"_oai":{"id":"oai:miyazaki-u.repo.nii.ac.jp:00000849","sets":["74","74:26"]},"author_link":["5389","2104","5390","932"],"control_number":"849","item_10001_alternative_title_1":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"β-carotene and fowl sperm function","subitem_alternative_title_language":"en"}]},"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2000","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"104","bibliographicPageStart":"99","bibliographicVolumeNumber":"56","bibliographic_titles":[{"bibliographic_title":"Molecular reproduction and development","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The motility of intact fowl spermatozoa was vigorous at 25°C, but decreased gradually following the addition of 0-100 μM β-carotene in a dose-dependent manner. Even in the presence of stimulators of fowl sperm motility, such as Ca2+ or calyculin A, the motility of intact spermatozoa at both 25°C and 40°C remained inhibited following the addition of β- carotene. Under all of these circumstances, sperm ATP concentrations were not reduced by the addition of β-carotene. Moreover, the motility of demembranated spermatozoa was not inhibited by the addition of the same concentrations of β-carotene. No changes in intracellular free Ca2+ concentrations, measured by means of a fluorescent Ca2+ indicator, fura-2, were observed in intact β-carotene -treated spermatozoa. These results suggest that β-carotene is involved in the inhibition of the flagellar movement of fowl spermatozoa without change in energy production, and that the target of β-carotene might be present in the cytoplasmic matrix and/or the plasma membrane, but not retained in the axoneme and/or accessory cytoskeletal components.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA10735246","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1040452X","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"Ashizawa, Koji","creatorNameLang":"en"},{"creatorName":"芦沢, 幸二","creatorNameLang":"ja"},{"creatorName":"アシザワ, コウジ","creatorNameLang":"ja-Kana"}],"familyNames":[{"familyName":"Ashizawa","familyNameLang":"en"},{"familyName":"芦沢","familyNameLang":"ja"},{"familyName":"アシザワ","familyNameLang":"ja-Kana"}],"givenNames":[{"givenName":"Koji","givenNameLang":"en"},{"givenName":"幸二","givenNameLang":"ja"},{"givenName":"コウジ","givenNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"932","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tomita, Yoshifumi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"5389","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kanemaru, Chikako","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"5390","nameIdentifierScheme":"WEKO"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"0000000106573887","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"https://isni.org/isni/0000000106573887"}],"affiliationNames":[{"affiliationName":"宮崎大学","affiliationNameLang":"ja"},{"affiliationName":"University of Miyazaki","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"續木, 靖浩","creatorNameLang":"ja"},{"creatorName":"ツヅキ, ヤスヒロ","creatorNameLang":"ja-Kana"},{"creatorName":"Tsuzuki, Yasuhiro","creatorNameLang":"en"}],"familyNames":[{"familyName":"續木","familyNameLang":"ja"},{"familyName":"ツヅキ","familyNameLang":"ja-Kana"},{"familyName":"Tsuzuki","familyNameLang":"en"}],"givenNames":[{"givenName":"靖浩","givenNameLang":"ja"},{"givenName":"ヤスヒロ","givenNameLang":"ja-Kana"},{"givenName":"Yasuhiro","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"2104","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"00236928","nameIdentifierScheme":"e-Rad_Researcher","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=00236928"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-06-21"}],"displaytype":"detail","filename":"ashizawa73.pdf","filesize":[{"value":"1.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"ashizawa73.pdf","url":"https://miyazaki-u.repo.nii.ac.jp/record/849/files/ashizawa73.pdf"},"version_id":"5d4965f3-acca-4bae-8cef-bbb658fd7f01"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"精子","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"sperm, Spermatozoa, β-carotene, Motility, ATP concentrations, Intracellular free calcium concentrations","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"In vitro Effects of β-Carotene for the Motility, ATP and Intracellular Free Ca2+ Concentrations of Fowl Spermatozoa","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"In vitro Effects of β-Carotene for the Motility, ATP and Intracellular Free Ca2+ Concentrations of Fowl Spermatozoa","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"5","path":["74","26"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2007-10-11"},"publish_date":"2007-10-11","publish_status":"0","recid":"849","relation_version_is_last":true,"title":["In vitro Effects of β-Carotene for the Motility, ATP and Intracellular Free Ca2+ Concentrations of Fowl Spermatozoa"],"weko_creator_id":"5","weko_shared_id":-1},"updated":"2024-11-22T06:53:23.089064+00:00"}