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Fundamental and distinct ways for irreversible creation of chromatin structure plasticity of proximal 5'-upstream regions of Pax5, Aiolos, EBF1 and OBF1 genes with epigenetic modifications for gain of new cell function to exclude accumulated IgM H- and L-chains in individual clones of HDAC2(-/-) DT40 mutants through various generations during continuous cultivation
http://hdl.handle.net/10458/6505
http://hdl.handle.net/10458/6505c1fd53ce-3ab4-4025-a295-ddc4c60a7ed9
| Item type | その他 / Others(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2020-06-21 | |||||
| タイトル | ||||||
| タイトル | Fundamental and distinct ways for irreversible creation of chromatin structure plasticity of proximal 5'-upstream regions of Pax5, Aiolos, EBF1 and OBF1 genes with epigenetic modifications for gain of new cell function to exclude accumulated IgM H- and L-chains in individual clones of HDAC2(-/-) DT40 mutants through various generations during continuous cultivation | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Irreversible creation of chromatin structure plasticity, epigenetic modifications, gain of new cell function, continuous cultivation, neighboring overlapping tiling chromatin immuno-precipitation (NotchIP) assay, changes in acetylation levels of specific Lys residues of histone H3 during cultivation, proximal 5'-upstream chromatin regions of specific transcription factor genes | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_1843 | |||||
| 資源タイプ | other | |||||
| 著者 |
中山, 雅美
× 中山, 雅美× 中山, 建男 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | We studied acetylation levels of Lys-9, Lys-14, Lys-18, Lys-23 and Lys-27 residues of histone H3 surrounding proximal ~2.0 kb 5'-upstream chromatin regions of Pax5, Aiolos, EBF1, OBF1 and PCAF genes in four individual clones （cl.2-1, cl.2-2, cl.2-4 and cl.2-6） of chicken histone deacetylase-2 （HDAC2）-deficient DT40 mutants HDAC2（-/-） at the early, middle and later cultivation stages. Acetylation levels of the five specific Lys residues of these transcription factor and chromatin-modifying enzyme genes were high in DT40 cells. In clone cl.2-1, acetylation levels of one or more of the Lys residues of Pax5, Aiolos and EBF1 genes were dramatically decreased at the early stage and remained unchanged until the later stage, and those of the OBF1 gene were drastically decreased until the later stage. In clones cl.2-2 and cl.2-4, acetylation levels of Pax5, Aiolos and EBF1 genes were dramatically decreased at the early stage, and those of the first two were increased until the later stage but those of the last one remained unchanged. In clone cl.2-6, acetylation levels of Pax5, Aiolos and EBF1 genes were drastically decreased at the early stage and thereafter increased until the later stage. These results could explain the previously mentioned ways for varied gene expressions of Pax5, Aiolos, EBF1 and OBF1 in individual HDAC2（-/-） clones during cultivation. We propose a hypothesis concerning distinct ways for gain of new cell function to eliminate accumulated IgM H- and L-chains in individual HDAC2（-/-） clones during continuous cultivation. They have an ability to adapt themselves to new environments by means of irreversible creation of the chromatin structure plasticity caused by successive conformational （structural） changes between the tight and loose forms depending on hypo- and hyper-acetylation levels of specific Lys residues of histone H3 surrounding proximal ~2.0 kb 5'-upstream chromatin regions of corresponding genes through various generations, indicating that DT40 cells are pluri-potent, elastic and flexible for gaining new cell function, attributed to alterations in the chromatin structure. | |||||
| 言語 | en | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | The original edition of the revision is available from following URL: http://opac2.lib.miyazaki-u.ac.jp/webopac/TC10145181 | |||||
| 言語 | en | |||||
| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
