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Fundamental and distinct ways for irreversible creation of chromatin structure plasticity of proximal 5'-upstream regions of Pax5, Aiolos, EBF1 and OBF1 genes with epigenetic modifications for gain of new cell function to exclude accumulated IgM H- and L-chains in individual clones of HDAC2(-/-) DT40 mutants through various generations during continuous cultivation

http://hdl.handle.net/10458/6505
http://hdl.handle.net/10458/6505
9fbbb305-7cdf-4e01-bc28-bc388bdd5d9a
名前 / ファイル ライセンス アクション
4_1_text_of_paper_4_revision.pdf text (525.6 kB)
1-Supplementary Supplementary Remarks (61.5 kB)
fig1.pdf fig1 (1.9 MB)
fig2.pdf fig2 (1.9 MB)
fig3.pdf fig3 (2.5 MB)
fig4.pdf fig4 (2.2 MB)
fig5.pdf fig5 (2.3 MB)
fig6.pdf fig6 (2.3 MB)
fig7.pdf fig7 (2.3 MB)
fig8.pdf fig8 (2.3 MB)
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fig11.pdf fig11 (3.0 MB)
fig12.pdf fig12 (2.9 MB)
fig13.pdf fig13 (2.4 MB)
fig14.pdf fig14 (2.3 MB)
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fig17.pdf fig17 (2.3 MB)
fig18.pdf fig18 (2.3 MB)
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fig20.pdf fig20 (2.2 MB)
fig21.pdf fig21 (432.2 kB)
tablei.pdf TableI (464.1 kB)
tableii.pdf TablelI (78.8 kB)
tableiii.pdf TableIll (84.9 kB)
tableiv.pdf TableIV (80.8 kB)
tablev.pdf TableV (83.8 kB)
Item type その他 / Others(1)
公開日 2020-06-21
タイトル
タイトル Fundamental and distinct ways for irreversible creation of chromatin structure plasticity of proximal 5'-upstream regions of Pax5, Aiolos, EBF1 and OBF1 genes with epigenetic modifications for gain of new cell function to exclude accumulated IgM H- and L-chains in individual clones of HDAC2(-/-) DT40 mutants through various generations during continuous cultivation
言語 en
言語
言語 eng
キーワード
言語 en
主題Scheme Other
主題 Irreversible creation of chromatin structure plasticity, epigenetic modifications, gain of new cell function, continuous cultivation, neighboring overlapping tiling chromatin immuno-precipitation (NotchIP) assay, changes in acetylation levels of specific Lys residues of histone H3 during cultivation, proximal 5'-upstream chromatin regions of specific transcription factor genes
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_1843
資源タイプ other
著者 中山, 雅美

× 中山, 雅美

WEKO 23623

ja 中山, 雅美

ja-Kana ナカヤマ, マサミ

en Nakayama, Masami

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中山, 建男

× 中山, 建男

WEKO 4839

ja 中山, 建男

ja-Kana ナカヤマ, タツオ

en Nakayama, Tatsuo

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抄録
内容記述タイプ Abstract
内容記述 We studied acetylation levels of Lys-9, Lys-14, Lys-18, Lys-23 and Lys-27 residues of histone H3 surrounding proximal ~2.0 kb 5'-upstream chromatin regions of Pax5, Aiolos, EBF1, OBF1 and PCAF genes in four individual clones (cl.2-1, cl.2-2, cl.2-4 and cl.2-6) of chicken histone deacetylase-2 (HDAC2)-deficient DT40 mutants HDAC2(-/-) at the early, middle and later cultivation stages. Acetylation levels of the five specific Lys residues of these transcription factor and chromatin-modifying enzyme genes were high in DT40 cells. In clone cl.2-1, acetylation levels of one or more of the Lys residues of Pax5, Aiolos and EBF1 genes were dramatically decreased at the early stage and remained unchanged until the later stage, and those of the OBF1 gene were drastically decreased until the later stage. In clones cl.2-2 and cl.2-4, acetylation levels of Pax5, Aiolos and EBF1 genes were dramatically decreased at the early stage, and those of the first two were increased until the later stage but those of the last one remained unchanged. In clone cl.2-6, acetylation levels of Pax5, Aiolos and EBF1 genes were drastically decreased at the early stage and thereafter increased until the later stage. These results could explain the previously mentioned ways for varied gene expressions of Pax5, Aiolos, EBF1 and OBF1 in individual HDAC2(-/-) clones during cultivation. We propose a hypothesis concerning distinct ways for gain of new cell function to eliminate accumulated IgM H- and L-chains in individual HDAC2(-/-) clones during continuous cultivation. They have an ability to adapt themselves to new environments by means of irreversible creation of the chromatin structure plasticity caused by successive conformational (structural) changes between the tight and loose forms depending on hypo- and hyper-acetylation levels of specific Lys residues of histone H3 surrounding proximal ~2.0 kb 5'-upstream chromatin regions of corresponding genes through various generations, indicating that DT40 cells are pluri-potent, elastic and flexible for gaining new cell function, attributed to alterations in the chromatin structure.
言語 en
内容記述
内容記述タイプ Other
内容記述 The original edition of the revision is available from following URL: http://opac2.lib.miyazaki-u.ac.jp/webopac/TC10145181
言語 en
著者版フラグ
出版タイプ AM
出版タイプResource http://purl.org/coar/version/c_ab4af688f83e57aa
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Ver.3 2023-07-30 07:18:53.750214
Ver.2 2023-07-30 06:07:30.477871
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中山, 雅美, 中山, 建男, n.d., Fundamental and distinct ways for irreversible creation of chromatin structure plasticity of proximal 5'-upstream regions of Pax5, Aiolos, EBF1 and OBF1 genes with epigenetic modifications for gain of new cell function to exclude accumulated IgM H- and L-chains in individual clones of HDAC2(-/-) DT40 mutants through various generations during continuous cultivation.

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