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DEPHOSPHORYLATION OF A 30 KDa PROTEIN OF FOWL SPERMATOZOA BY THE ADDITION OF MYOSIN LIGHT CHAIN KINASE SUBSTRATE PEPTIDE INHIBITS THE FLAGELLAR MOTILITY
http://hdl.handle.net/10458/719
http://hdl.handle.net/10458/719095c9d29-738f-4034-926d-2e1658bf006e
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
ashizawa56.pdf (788.4 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2007-10-10 | |||||
| タイトル | ||||||
| タイトル | DEPHOSPHORYLATION OF A 30 KDa PROTEIN OF FOWL SPERMATOZOA BY THE ADDITION OF MYOSIN LIGHT CHAIN KINASE SUBSTRATE PEPTIDE INHIBITS THE FLAGELLAR MOTILITY | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | ja | |||||
| キーワード | 精子 | |||||
| キーワード | ||||||
| 言語 | en | |||||
| キーワード | sperm | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| 著者 |
芦沢, 幸二
× 芦沢, 幸二× Wishart, Graham J.× Hashimoto, Kei× Tsuzuki, Yasuhiro |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Phosphorylation of demembranated fowl sperm proteins during incubation with [γ-32P]ATP and various protein kinase substrate peptides at 30°C was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis （SDS-PAGE）. A marked difference in phosphorylation was observed in a 30 kDa protein. This protein was strongly phosphorylated after the addition of Kemptide, a cAMP-dependent protein kinase （PKA） substrate peptide; Syntide 2, a calmodulin-dependent protein kinase II substrate peptide; a protein kinase C （PKC） substrate peptide; as well as control samples but only slightly phosphorylated in the presence of a myosin light chain kinase （MLCK） substrate peptide. The motility of demembranated spermatozoa at 30°C remained high in control samples and following the addition of Kemptide, Syntide 2 and PKC substrate peptide, but decreased markedly following the addition of MLCK substrate peptide. These results suggest that the 30 kDa protein is identified as a substrate for MLCK or a MLCK-like protein in fowl spermatozoa and that phosphorylation-dephosphorylation of this protein is involved in the regulation of flagellar movement at 30°C. | |||||
| 言語 | en | |||||
| 書誌情報 |
en : Biochemical and biophysical research communications 巻 215, 号 2, p. 706-712, 発行日 1995 |
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| 出版者 | ||||||
| 出版者 | Elsevier | |||||
| 言語 | en | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0006291X | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA00564395 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
