{"created":"2023-05-15T12:41:00.776851+00:00","links":{},"metadata":{"_buckets":{"deposit":"d88dbd13-e908-466a-b203-f53b865a9cdb"},"_deposit":{"id":"538.1","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"538.1"},"status":"published"},"_oai":{"id":"oai:miyazaki-u.repo.nii.ac.jp:00000538.1","sets":["74:26"]},"author_link":["932","3134","3135","2104"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1995","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"712","bibliographicPageStart":"706","bibliographicVolumeNumber":"215","bibliographic_titles":[{"bibliographic_title":"Biochemical and biophysical research communications","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Phosphorylation of demembranated fowl sperm proteins during incubation with [γ-32P]ATP and various protein kinase substrate peptides at 30°C was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis （SDS-PAGE）. A marked difference in phosphorylation was observed in a 30 kDa protein. This protein was strongly phosphorylated after the addition of Kemptide, a cAMP-dependent protein kinase （PKA） substrate peptide; Syntide 2, a calmodulin-dependent protein kinase II substrate peptide; a protein kinase C （PKC） substrate peptide; as well as control samples but only slightly phosphorylated in the presence of a myosin light chain kinase （MLCK） substrate peptide. The motility of demembranated spermatozoa at 30°C remained high in control samples and following the addition of Kemptide, Syntide 2 and PKC substrate peptide, but decreased markedly following the addition of MLCK substrate peptide. These results suggest that the 30 kDa protein is identified as a substrate for MLCK or a MLCK-like protein in fowl spermatozoa and that phosphorylation-dephosphorylation of this protein is involved in the regulation of flagellar movement at 30°C.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier","subitem_publisher_language":"en"}]},"item_10001_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00564395","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0006291X","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"芦沢, 幸二","creatorNameLang":"ja"},{"creatorName":"Ashizawa, Koji","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"932","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Wishart, Graham J.","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"3134","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hashimoto, Kei","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"3135","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tsuzuki, Yasuhiro","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"2104","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"00236928","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=00236928"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-06-21"}],"displaytype":"detail","filename":"ashizawa56.pdf","filesize":[{"value":"788.4 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"ashizawa56.pdf","url":"https://miyazaki-u.repo.nii.ac.jp/record/538.1/files/ashizawa56.pdf"},"version_id":"2830282a-17ba-437f-9aea-7333b5c47469"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"精子","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"sperm","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"DEPHOSPHORYLATION OF A 30 KDa PROTEIN OF FOWL SPERMATOZOA BY THE ADDITION OF MYOSIN LIGHT CHAIN KINASE SUBSTRATE PEPTIDE INHIBITS THE FLAGELLAR MOTILITY","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"DEPHOSPHORYLATION OF A 30 KDa PROTEIN OF FOWL SPERMATOZOA BY THE ADDITION OF MYOSIN LIGHT CHAIN KINASE SUBSTRATE PEPTIDE INHIBITS THE FLAGELLAR MOTILITY","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"2","path":["26"],"pubdate":{"attribute_name":"公開日","attribute_value":"2007-10-10"},"publish_date":"2007-10-10","publish_status":"0","recid":"538.1","relation_version_is_last":true,"title":["DEPHOSPHORYLATION OF A 30 KDa PROTEIN OF FOWL SPERMATOZOA BY THE ADDITION OF MYOSIN LIGHT CHAIN KINASE SUBSTRATE PEPTIDE INHIBITS THE FLAGELLAR MOTILITY"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-07-21T09:27:08.461784+00:00"}