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  1. 農学部
  1. 農学部
  2. 学術雑誌掲載論文 (農学部)

Improvement of the post-thaw qualities of Okinawan native pig spermatozoa frozen in an extender supplemented with ascorbic acid 2-O-α-glucoside

http://hdl.handle.net/10458/1906
http://hdl.handle.net/10458/1906
ebefba9e-ae4b-4938-963c-d0b64add0043
名前 / ファイル ライセンス アクション
Cryobiology-2008.pdf Cryobiology-2008.pdf (948.7 kB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2009-03-02
タイトル
タイトル Improvement of the post-thaw qualities of Okinawan native pig spermatozoa frozen in an extender supplemented with ascorbic acid 2-O-α-glucoside
言語 en
言語
言語 eng
キーワード
言語 en
主題Scheme Other
キーワード AA-2G, Agu, cell, damage, cryopreservation, oxidative stress, spermatozoa
資源タイプ
資源タイプ journal article
著者 芦沢, 幸二

× 芦沢, 幸二

WEKO 932

ja 芦沢, 幸二

en Ashizawa, Koji

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Yoshimoto, Teppei

× Yoshimoto, Teppei

WEKO 3049

en Yoshimoto, Teppei

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Nakamura, Satoshi

× Nakamura, Satoshi

WEKO 3050

en Nakamura, Satoshi

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Yamauchi, Shogo

× Yamauchi, Shogo

WEKO 3051

en Yamauchi, Shogo

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Muto, Norio

× Muto, Norio

WEKO 3052

en Muto, Norio

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Nakada, Tadashi

× Nakada, Tadashi

WEKO 3053

en Nakada, Tadashi

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Tatemoto, Hideki

× Tatemoto, Hideki

WEKO 3055

en Tatemoto, Hideki

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抄録
内容記述タイプ Abstract
内容記述 The technical establishment of boar sperm cryopreservation is indispensable for effective breeding of the scarce Okinawan native pig Agu. The objective of the present study was to determine whether ascorbic acid 2-O-α-glucoside (AA-2G), a stable ascorbate derivative, is capable of improving the quality of cryopreserved Agu spermatozoa. Ejaculated Agu sperm frozen in an extender supplemented with 0, 100, 200, 400 or 800 μM AA-2G was thawed, and then evaluated the sperm motility and other qualities. Treatment with 200 μM AA-2G has the most beneficial effect on the sperm motility and the plasmalemma integrity after frozen-thawing among the concentrations tested (P<0.05). In particular, the incidences of total motile sperm and rapid progressive motility at 1 and 3 h after incubation were markedly increased by treatment with AA-2G at 200 μM. The addition of AA-2G during cooling and freezing efficiently protected spermatozoa against the lipid peroxidation and the DNA damage. Spermatozoa frozen in the presence of AA-2G possessed significantly higher levels (P<0.05) of ATP even after thawing than those frozen without AA-2G, implying that sperm viability was effectively conserved. Furthermore,
higher sperm penetrability to matured oocytes in vitro was maintained in sperm treated with AA-2G during cryopreservation. These effects were observed for all sperm derived from three individuals. These findings demonstrate that the addition of AA-2G to the freezing extender efficiently improves the post-thaw qualities of fragile Agu sperm through the protection of spermatozoa against cell damage caused by oxidative stress during
cryopreservation.
言語 en
書誌情報 en : Cryobiology : journal of the Society for Cryobiology

巻 57, 号 1, p. 30-36, 発行日 2008-08
出版者
出版者 Elsevier
言語 en
ISSN
収録物識別子タイプ ISSN
収録物識別子 00112240
ISSN
収録物識別子タイプ ISSN
収録物識別子 10902392
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA00620601
著者版フラグ
出版タイプ AM
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