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Improvement of the post-thaw qualities of Okinawan native pig spermatozoa frozen in an extender supplemented with ascorbic acid 2-O-α-glucoside
http://hdl.handle.net/10458/1906
http://hdl.handle.net/10458/190682641e14-4aee-4fc8-88c2-ff3c2d79fcb2
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
Cryobiology-2008.pdf (948.7 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2009-03-02 | |||||
| タイトル | ||||||
| タイトル | Improvement of the post-thaw qualities of Okinawan native pig spermatozoa frozen in an extender supplemented with ascorbic acid 2-O-α-glucoside | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | AA-2G, Agu, cell, damage, cryopreservation, oxidative stress, spermatozoa | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| 著者 |
芦沢, 幸二
× 芦沢, 幸二× Yoshimoto, Teppei× Nakamura, Satoshi× Yamauchi, Shogo× Muto, Norio× Nakada, Tadashi× 建本, 秀樹 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The technical establishment of boar sperm cryopreservation is indispensable for effective breeding of the scarce Okinawan native pig Agu. The objective of the present study was to determine whether ascorbic acid 2-O-α-glucoside （AA-2G）, a stable ascorbate derivative, is capable of improving the quality of cryopreserved Agu spermatozoa. Ejaculated Agu sperm frozen in an extender supplemented with 0, 100, 200, 400 or 800 μM AA-2G was thawed, and then evaluated the sperm motility and other qualities. Treatment with 200 μM AA-2G has the most beneficial effect on the sperm motility and the plasmalemma integrity after frozen-thawing among the concentrations tested （P<0.05）. In particular, the incidences of total motile sperm and rapid progressive motility at 1 and 3 h after incubation were markedly increased by treatment with AA-2G at 200 μM. The addition of AA-2G during cooling and freezing efficiently protected spermatozoa against the lipid peroxidation and the DNA damage. Spermatozoa frozen in the presence of AA-2G possessed significantly higher levels （P<0.05） of ATP even after thawing than those frozen without AA-2G, implying that sperm viability was effectively conserved. Furthermore, higher sperm penetrability to matured oocytes in vitro was maintained in sperm treated with AA-2G during cryopreservation. These effects were observed for all sperm derived from three individuals. These findings demonstrate that the addition of AA-2G to the freezing extender efficiently improves the post-thaw qualities of fragile Agu sperm through the protection of spermatozoa against cell damage caused by oxidative stress during cryopreservation. |
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| 言語 | en | |||||
| 書誌情報 |
en : Cryobiology : journal of the Society for Cryobiology 巻 57, 号 1, p. 30-36, 発行日 2008-08 |
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| 出版者 | ||||||
| 出版者 | Elsevier | |||||
| 言語 | en | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 00112240 | |||||
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| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 10902392 | |||||
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| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA00620601 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
