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Purification and Properties of an Extracellular β-Xylosidase from Aspergillus japonicus and Sequence Analysis of the Encoding Gene
http://hdl.handle.net/10458/1791
http://hdl.handle.net/10458/17915b5df6b3-5f43-4f00-8207-8c5d59dab424
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
jbb106398.pdf (2.2 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2009-02-03 | |||||
| タイトル | ||||||
| タイトル | Purification and Properties of an Extracellular β-Xylosidase from Aspergillus japonicus and Sequence Analysis of the Encoding Gene | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Aspergillus japonicus, β-xylosidase, extracellular enzyme, xylan | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Wakiyama, Motoki
× Wakiyama, Motoki× Yoshihara, Koji× Hayashi, Sachio× Ohta, Kazuyoshi |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | An extracellular protein exhibiting β-xylosidase activity was purified from the culture filtrate of a filamentous fungus, Aspergillus japonicus strain MU-2, grown on oat spelt xylan. The purified enzyme was a monomeric glycoprotein with an apparent Mr of 113.2 kDa as estimated by SDS-PAGE. β-Xylosidase activity was optimal at pH 4.0 and 70°C. The enzyme also showed β-glucosidase and α-l-arabinofuranosidase activities. The genomic DNA and cDNA encoding this protein were cloned and sequenced. Southern blot analysis indicated that the β-xylosidase gene （xylA） was present as a single copy in the genome. An open reading frame, consisting of 2412 bp, was not interrupted by introns, and it encoded a presumed signal peptide of 17 amino acids and a mature protein of 787 amino acids. The deduced amino acid sequence of the xylA gene product showed a high degree of identity （69%） to the primary structure of the Aspergillus niger β-xylosidase XlnD that belongs to the glycoside hydrolase family 3. Moreover, the xylA gene was functionally expressed in the yeast Pichia pastoris. | |||||
| 言語 | en | |||||
| 書誌情報 |
en : Journal of bioscience and bioengineering 巻 106, 号 4, p. 398-404, 発行日 2008-10 |
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| 出版者 | ||||||
| 出版者 | 日本生物工学会 | |||||
| 言語 | ja | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 13891723 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA11307678 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
