{"created":"2023-05-15T12:41:13.227768+00:00","links":{},"metadata":{"_buckets":{"deposit":"41d300cb-58a0-46fc-bab5-21e0d82ac9b2"},"_deposit":{"id":"520.1","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"520.1"},"status":"published"},"_oai":{"id":"oai:miyazaki-u.repo.nii.ac.jp:00000520.1","sets":["74:26"]},"author_link":["3032","3033","3034","2632"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-10","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"404","bibliographicPageStart":"398","bibliographicVolumeNumber":"106","bibliographic_titles":[{"bibliographic_title":"Journal of bioscience and bioengineering","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"An extracellular protein exhibiting β-xylosidase activity was purified from the culture filtrate of a filamentous fungus, Aspergillus japonicus strain MU-2, grown on oat spelt xylan. The purified enzyme was a monomeric glycoprotein with an apparent Mr of 113.2 kDa as estimated by SDS-PAGE. β-Xylosidase activity was optimal at pH 4.0 and 70°C. The enzyme also showed β-glucosidase and α-l-arabinofuranosidase activities. The genomic DNA and cDNA encoding this protein were cloned and sequenced. Southern blot analysis indicated that the β-xylosidase gene （xylA） was present as a single copy in the genome. An open reading frame, consisting of 2412 bp, was not interrupted by introns, and it encoded a presumed signal peptide of 17 amino acids and a mature protein of 787 amino acids. The deduced amino acid sequence of the xylA gene product showed a high degree of identity （69%） to the primary structure of the Aspergillus niger β-xylosidase XlnD that belongs to the glycoside hydrolase family 3. Moreover, the xylA gene was functionally expressed in the yeast Pichia pastoris.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"日本生物工学会","subitem_publisher_language":"ja"}]},"item_10001_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11307678","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13891723","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Wakiyama, Motoki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"3032","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yoshihara, Koji","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"3033","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hayashi, Sachio","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"3034","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ohta, Kazuyoshi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"2632","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-06-21"}],"displaytype":"detail","filename":"jbb106398.pdf","filesize":[{"value":"2.2 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"jbb106398.pdf","url":"https://miyazaki-u.repo.nii.ac.jp/record/520.1/files/jbb106398.pdf"},"version_id":"bd5aac9c-ba0f-4b68-8723-491eea522e54"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Aspergillus japonicus, β-xylosidase, extracellular enzyme, xylan","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Purification and Properties of an Extracellular β-Xylosidase from Aspergillus japonicus and Sequence Analysis of the Encoding Gene","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Purification and Properties of an Extracellular β-Xylosidase from Aspergillus japonicus and Sequence Analysis of the Encoding Gene","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"2","path":["26"],"pubdate":{"attribute_name":"公開日","attribute_value":"2009-02-03"},"publish_date":"2009-02-03","publish_status":"0","recid":"520.1","relation_version_is_last":true,"title":["Purification and Properties of an Extracellular β-Xylosidase from Aspergillus japonicus and Sequence Analysis of the Encoding Gene"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-07-21T09:31:07.006728+00:00"}