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Naïve-like conversion enhances the difference in innate in vitro differentiation capacity between rabbit ES cells and iPS cells
http://hdl.handle.net/10458/5429
http://hdl.handle.net/10458/5429b16f5000-02fa-4b8f-8cdc-a427b3cf0b06
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2020-06-21 | |||||
| タイトル | ||||||
| タイトル | Naïve-like conversion enhances the difference in innate in vitro differentiation capacity between rabbit ES cells and iPS cells | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | Embryonic stem cells (ESCs), Induced pluripotent stem cells (iPSCs), Naïve, Neural differentiation, Rabbit | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| 著者 |
本勝, 希実子
× 本勝, 希実子× 廣瀬, 美智子× Hatori, Masanori× Yasmin, Lubna× 伊豆, 美奈× 的場, 章悟× Togayachi, Sumie× 三好, 浩之× 山海, 直× 小倉, 淳郎× 本多, 新 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Quality evaluation of pluripotent stem cells using appropriate animal models needs to be improved for human regenerative medicine. Previously, we demonstrated that although the in vitro neural differentiating capacity of rabbit induced pluripotent stem cells （iPSCs） can be mitigated by improving their baseline level of pluripotency, i.e., by converting them into the so-called “naïve-like" state, the effect after such conversion of rabbit embryonic stem cells （ESCs） remains to be elucidated. Here we found that naïve-like conversion enhanced the differences in innate in vitro differentiation capacity between ESCs and iPSCs. Naïve-like rabbit ESCs exhibited several features indicating pluripotency, including the capacity for teratoma formation. They differentiated into mature oligodendrocytes much more effectively （3.3–7.2 times） than naïve-like iPSCs. This suggests an inherent variation in differentiation potential in vitro among PSC lines. When naïve-like ESCs were injected into preimplantation rabbit embryos, although they contributed efficiently to forming the inner cell mass of blastocysts, no chimeric pups were obtained. Thus, in vitro neural differentiation following naïve-like conversion is a promising option for determining the quality of PSCs without the need to demonstrate chimeric contribution. These results provide an opportunity to evaluate which pluripotent stem cells or treatments are best suited for therapeutic use. | |||||
| 言語 | en | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 本論文に関連する研究論文 Honsho K, Hirose M, Hatori M, Yasmin L, Izu H, Matoba S, Togayachi S, Miyoshi H, Sankai T, Ogura A, Honda A. Naïve-like conversion enhances the difference in innate in vitro differentiation capacity between rabbit ES cells and iPS cells. J Reprod Dev. 2015;61(1):13-9. doi: 10.1262/jrd.2014-098. Epub 2014 Oct 25. PMID: 25345855; PMCID: PMC4354226. |
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| 言語 | ja | |||||
| 書誌情報 |
The Journal of reproduction and development 巻 61, 号 1, p. 13-19, 発行日 2015-02 |
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| 出版者 | ||||||
| 出版者 | 日本繁殖生物学会 | |||||
| 言語 | ja | |||||
| 出版者 | ||||||
| 出版者 | Society for Reproduction and Development | |||||
| 言語 | en | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 09168818 | |||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1262/jrd.2014-098 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | NA | |||||
