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A Protein Extraction System with Water/Oil Microemulsion Formed by a Biodegradable Polymer Surfactant
http://hdl.handle.net/10458/5021
http://hdl.handle.net/10458/5021340feba1-83f8-4bef-86d0-5d054cdf7308
| 名前 / ファイル | ライセンス | アクション |
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本文 (810.0 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||
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| 公開日 | 2020-06-21 | |||||||||||||
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| タイトル | A Protein Extraction System with Water/Oil Microemulsion Formed by a Biodegradable Polymer Surfactant | |||||||||||||
| 言語 | en | |||||||||||||
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| 言語 | eng | |||||||||||||
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| 資源タイプ | journal article | |||||||||||||
| 著者 |
西野, 悟
× 西野, 悟× 岸田, 晶夫× 吉澤, 秀和× 塩盛, 弘一郎
WEKO
5570
× Nishino, Satoru× Kishida, Akio× 吉澤, 秀和 |
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| 抄録 | ||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||
| 内容記述 | The amphiphilic biodegradable polymer surfactant, poly（ethyleneoxide monooleate）-block- poly（D,L-lactide） （MOPEO-PLA）, was shown to form microemulsions in chloroform solution and to extract water and protein into their microemulsions from the aqueous phase. The water content in the chloroform phase significantly increased with the MOPEO-PLA concentration, indicating that a MOPEO-PLA microemulsion was formed with a large amount of water molecules. The largest amount of cytochrome c in the MOPEO-PLA microemulsion phase was extracted when the pH of the aqueous phase was close to the pI value for cytochrome c, and the degree of extraction increased at higher temperatures. From these results, the main factor for the extraction of cytochrome c was found to be the hydrophobic interaction between MOPEO-PLA and cytochrome c. The MOPEO-PLA microemulsion provides a benign extraction process for proteins. A MOPEO-PLA microemulsion system would provide a new encapsulation system in a biodegradable polymer matrix for delivery of proteins and peptides. | |||||||||||||
| 書誌情報 |
Solvent Extraction Research and Development, Japan 巻 21, 号 1, p. 47-54, 発行日 2014-05 |
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| 出版者 | Japan Association of Solvent Extraction | |||||||||||||
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| 出版者 | JASE | |||||||||||||
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| 出版者 | 日本溶媒抽出学会 | |||||||||||||
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| 収録物識別子タイプ | ISSN | |||||||||||||
| 収録物識別子 | 21884765 | |||||||||||||
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| 収録物識別子タイプ | ISSN | |||||||||||||
| 収録物識別子 | 13417215 | |||||||||||||
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| 出版タイプ | VoR | |||||||||||||
