@article{oai:miyazaki-u.repo.nii.ac.jp:00004161, author = {西野, 悟 and 岸田, 晶夫 and Yoshizawa, Hidekazu and 吉澤, 秀和 and Shiomori, Koichiro and 塩盛, 弘一郎 and Shiomori, Kouichiro and Nishino, Satoru and Kishida, Akio and Yoshizawa, Hidekazu and 吉澤, 秀和}, issue = {1}, journal = {Solvent Extraction Research and Development, Japan}, month = {May}, note = {The amphiphilic biodegradable polymer surfactant, poly(ethyleneoxide monooleate)-block- poly(D,L-lactide) (MOPEO-PLA), was shown to form microemulsions in chloroform solution and to extract water and protein into their microemulsions from the aqueous phase. The water content in the chloroform phase significantly increased with the MOPEO-PLA concentration, indicating that a MOPEO-PLA microemulsion was formed with a large amount of water molecules. The largest amount of cytochrome c in the MOPEO-PLA microemulsion phase was extracted when the pH of the aqueous phase was close to the pI value for cytochrome c, and the degree of extraction increased at higher temperatures. From these results, the main factor for the extraction of cytochrome c was found to be the hydrophobic interaction between MOPEO-PLA and cytochrome c. The MOPEO-PLA microemulsion provides a benign extraction process for proteins. A MOPEO-PLA microemulsion system would provide a new encapsulation system in a biodegradable polymer matrix for delivery of proteins and peptides.}, pages = {47--54}, title = {A Protein Extraction System with Water/Oil Microemulsion Formed by a Biodegradable Polymer Surfactant}, volume = {21}, year = {2014}, yomi = {ニシノ, サトル and キシダ, アキオ and ヨシザワ , ヒデカズ and シオモリ, コウイチロウ and ヨシザワ , ヒデカズ} }