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  1. フロンティア科学実験総合センター
  1. フロンティア科学実験総合センター
  2. 学術雑誌掲載論文(フロンティア科学実験総合センター)

Essential role of chromatin assembly factor-1 -mediated rapid nucleosome assembly for DNA replication and cell division in vertebrate cells

http://hdl.handle.net/10458/4233
http://hdl.handle.net/10458/4233
1aa4ae16-d4c0-4f74-afc2-8d3e9572de40
名前 / ファイル ライセンス アクション
nakayama.pdf nakayama.pdf (1.9 MB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2012-12-18
タイトル
タイトル Essential role of chromatin assembly factor-1 -mediated rapid nucleosome assembly for DNA replication and cell division in vertebrate cells
言語 en
言語
言語 eng
資源タイプ
資源タイプ journal article
著者 中山, 建男

× 中山, 建男

WEKO 4839

中山, 建男

ja-Kana ナカヤマ, タツオ

en Nakayama, Tatsuo

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高見, 恭成

× 高見, 恭成

WEKO 16378
e-Rad 80236356

高見, 恭成

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Takami, Yasunari

× Takami, Yasunari

WEKO 16379

en Takami, Yasunari

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Ono, Tatsuya

× Ono, Tatsuya

WEKO 16380

en Ono, Tatsuya

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Fukagawa, Tatsuo

× Fukagawa, Tatsuo

WEKO 16381

en Fukagawa, Tatsuo

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Shibahara, Kei-Ichi

× Shibahara, Kei-Ichi

WEKO 16382

en Shibahara, Kei-Ichi

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抄録
内容記述タイプ Abstract
内容記述 Chromatin assembly factor-1 (CAF-1), a complex consisting of p150, p60, and p48 subunits, is highly conserved from yeast to humans and facilitates nucleosome assembly of newly replicated DNA in vitro. To investigate roles of CAF-1 in vertebrates, we generated two conditional DT40 mutants, respectively, devoid of CAF-1p150 and p60. Depletion of each of these CAF-1 subunits led to delayed S-phase progression concomitant with slow DNA synthesis, followed by accumulation in late S/G2 phase and aberrant mitosis associated with extra centrosomes, and then the final consequence was cell death. We demonstrated that CAF-1 is necessary for rapid nucleosome formation during DNA replication in vivo as well as in vitro. Loss of CAF-1 was not associated with the apparent induction of phosphorylations of S-checkpoint kinases Chk1 and Chk2. To elucidate the precise role of domain(s) in CAF-1p150, functional dissection analyses including rescue assays were preformed. Results showed that the binding abilities of CAF-1p150 with CAF-1p60 and DNA polymerase sliding clamp proliferating cell nuclear antigen (PCNA) but not with heterochromatin protein HP1-gamma are required for cell viability. These observations highlighted the essential role of CAF-1-dependent nucleosome assembly in DNA replication and cell proliferation through its interaction with PCNA.
書誌情報 Molecular biology of the cell

巻 18, 号 1, p. 129-141, 発行日 2007-01
ISSN
収録物識別子タイプ ISSN
収録物識別子 10591524
PubMed番号
関連タイプ isIdenticalTo
識別子タイプ PMID
関連識別子 17065558
権利
権利情報 ©2006 by The American Society for Cell Biology
著者版フラグ
出版タイプ VoR
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Nakayama, Tatsuo, 高見, 恭成, Takami, Yasunari, Ono, Tatsuya, Fukagawa, Tatsuo, Shibahara, Kei-Ichi, 2007, Essential role of chromatin assembly factor-1 -mediated rapid nucleosome assembly for DNA replication and cell division in vertebrate cells: 129–141 p.

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