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Essential role of chromatin assembly factor-1 -mediated rapid nucleosome assembly for DNA replication and cell division in vertebrate cells
http://hdl.handle.net/10458/4233
http://hdl.handle.net/10458/42338359645c-7e1c-4177-b1cf-2a5fff9d8bbd
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
nakayama.pdf (1.9 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2012-12-18 | |||||
| タイトル | ||||||
| タイトル | Essential role of chromatin assembly factor-1 -mediated rapid nucleosome assembly for DNA replication and cell division in vertebrate cells | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| 著者 |
中山, 建男
× 中山, 建男× 髙見, 恭成× 髙見, 恭成× Ono, Tatsuya× Fukagawa, Tatsuo× Shibahara, Kei-Ichi |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Chromatin assembly factor-1 （CAF-1）, a complex consisting of p150, p60, and p48 subunits, is highly conserved from yeast to humans and facilitates nucleosome assembly of newly replicated DNA in vitro. To investigate roles of CAF-1 in vertebrates, we generated two conditional DT40 mutants, respectively, devoid of CAF-1p150 and p60. Depletion of each of these CAF-1 subunits led to delayed S-phase progression concomitant with slow DNA synthesis, followed by accumulation in late S/G2 phase and aberrant mitosis associated with extra centrosomes, and then the final consequence was cell death. We demonstrated that CAF-1 is necessary for rapid nucleosome formation during DNA replication in vivo as well as in vitro. Loss of CAF-1 was not associated with the apparent induction of phosphorylations of S-checkpoint kinases Chk1 and Chk2. To elucidate the precise role of domain（s） in CAF-1p150, functional dissection analyses including rescue assays were preformed. Results showed that the binding abilities of CAF-1p150 with CAF-1p60 and DNA polymerase sliding clamp proliferating cell nuclear antigen （PCNA） but not with heterochromatin protein HP1-gamma are required for cell viability. These observations highlighted the essential role of CAF-1-dependent nucleosome assembly in DNA replication and cell proliferation through its interaction with PCNA. | |||||
| 書誌情報 |
Molecular biology of the cell 巻 18, 号 1, p. 129-141, 発行日 2007-01 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 10591524 | |||||
| PubMed番号 | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 17065558 | |||||
| 権利 | ||||||
| 権利情報 | ©2006 by The American Society for Cell Biology | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
