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ヒト腸管微生物叢のメタゲノム分析のためのDNA単離法の改良
http://hdl.handle.net/10458/4555
http://hdl.handle.net/10458/4555cfff2700-d94c-4a09-8122-e525212aa4d2
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
hayasi_1975.pdf (685.5 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2013-07-12 | |||||
| タイトル | ||||||
| タイトル | An Improved DNA Isolation Method for Metagenomic Analysis of the Microbial Flora of the Human Intestine | |||||
| 言語 | en | |||||
| タイトル | ||||||
| タイトル | ヒト腸管微生物叢のメタゲノム分析のためのDNA単離法の改良 | |||||
| 言語 | ja | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | metagenomics, DNA isolation, human feces, microbial flora | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| 著者 |
林, 哲也
× 林, 哲也× Morita, Hidetoshi× Kuwahara, Tomomi× Ohshima, Kenshiro× Sasamoto, Hiroyuki× Itoh, Kikuji× Hattori, Masahira× 林, 哲也× Takami, Hideto |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The efficiency with which lysis of five strictly anaerobic and six facultatively anaerobic bacterial species, all well-known human colonic commensals, were lysed was tested using a reference method for general metagenomic analysis and an improved method that involves higher levels of lysozyme and proteinase K, as well as the addition of achromopeptidase. Ten species were lysed with an efficiency of >80% by the reference method, while the lytic efficiency for Clostridium ramosum JCM 1298T was <50%. The lytic efficiency of the improved method for C. ramosum JCM 1298T was 82.5%. Similarly, five samples of human feces were tested with these methods, as well as with the QIAamp DNA stool mini kit. Although the efficiency of lysis of the microbes recovered from the fecal samples fluctuated depending on the sample in the cases of the reference method （13.3-84.6%） and QIAamp DNA stool mini kit （38.8-69.2%）, the improved method gave stable and high-level lysis （>90%） for all the fecal samples. Accordingly, since the DNA samples isolated by the improved method can reflect nearly true genomic information in the microbial flora, our improved method should be applicable to metagenomic analyses, not only for bacteria in the human intestine but also for bacteria in other environments. | |||||
| 書誌情報 |
Microbes and environments 巻 22, 号 3, p. 214-222, 発行日 2007-09 |
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| 出版者 | ||||||
| 出版者 | 日本土壌微生物学会 | |||||
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| 出版者 | JAPANESE SOCIETY OF SOIL MICROBIOLOGY | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 13426311 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
