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A water-soluble high molecular weight substance isolated from Hyuganatsu orange (Citrus tamurana), suspected to be a polysaccharide, inhibits rat osteoclast cell formation
http://hdl.handle.net/10458/0002000482
http://hdl.handle.net/10458/0002000482bd09a9e2-49d8-47fd-8c8a-1d0db8033924
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article (535 KB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||||||
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| 公開日 | 2024-02-03 | |||||||||||||||||
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| タイトル | A water-soluble high molecular weight substance isolated from Hyuganatsu orange (Citrus tamurana), suspected to be a polysaccharide, inhibits rat osteoclast cell formation | |||||||||||||||||
| 言語 | en | |||||||||||||||||
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| 言語 | eng | |||||||||||||||||
| キーワード | ||||||||||||||||||
| 言語 | en | |||||||||||||||||
| キーワード | Citrus Tamurana | |||||||||||||||||
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| 言語 | en | |||||||||||||||||
| キーワード | TRAP positive cell | |||||||||||||||||
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| 言語 | en | |||||||||||||||||
| キーワード | Polysaccharides | |||||||||||||||||
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| 言語 | en | |||||||||||||||||
| キーワード | Rat osteoclast cell | |||||||||||||||||
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| 言語 | en | |||||||||||||||||
| キーワード | in vitro study | |||||||||||||||||
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| 資源タイプ | journal article | |||||||||||||||||
| 著者 |
秦, 博子
× 秦, 博子× 山口, 昌俊
WEKO
20790
× 鮫島, 浩
WEKO
7804
× 池ノ上, 克× Matsubara, Junko× Tsuboi, Makoto× Tanaka, Takashi |
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| 内容記述タイプ | Abstract | |||||||||||||||||
| 内容記述 | Background: Osteoporosis is detrimental to aged women’s health care. We previously reported that Hyuganatsu orange (Citrus Tamurana) contains active substances that inhibit osteoclast activities. Prior to conducting a human study, we sought to identify the biological active substance in the Hyuganatsu orange which suppresses osteoclast formation. Methods: We isolated five fractions from a Hyuganatsu orange extract according to molecular weight. Each fraction was tested to determine its suppressive effect on the formation of osteoclasts in rats. We also used high-performance liquid chromatography (HPLC), infra-red (IR), and 1H and 13C NMR spectroscopy to evaluate its chemical structure. Data was recorded as mean ± standard error of the mean. The Mann-Whitney test was used, and a p-value of <.05 was considered statistically significant. Results: The highest and lowest molecular weight fractions showed significant suppression activity on rat osteoclast formation (p < .05). The lowest molecular weight fraction was identified as hesperidin using thin layer chromatography. Additionally, IR absorption revealed that the highest molecular weight fraction was not a flavonoid. With regard to chemical structure, 1H and 13C NMR spectroscopy suggested that the highest molecular weight fraction had signals compatible with a polysaccharide such as galacturonic acid. Conclusions: Hyuganatsu orange contains a biological active substance other than hesperidin that may be a polysaccharide and may suppress osteoclast formation. |
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| 言語 | en | |||||||||||||||||
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| 内容記述タイプ | Other | |||||||||||||||||
| 内容記述 | Citation: Hata, H., Yamaguchi, M., Sameshima, H., Ikenoue, T., Matsubara, J., Tsuboi, M., and Tanaka, T. (2015). A water-soluble high molecular weight substance isolated from Hyuganatsu orange (Citrus tamurana), suspected to be polysaccharide, inhibits rat osteoclast cell formation. Funct.. Foods Health Dis., 5, 188-199. |
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| 言語 | en | |||||||||||||||||
| 書誌情報 |
en : Functional Foods in Health and Disease 巻 5, 号 6, p. 188 |
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| 言語 | en | |||||||||||||||||
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| 収録物識別子タイプ | ISSN | |||||||||||||||||
| 収録物識別子 | 23787007 | |||||||||||||||||
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| 関連タイプ | isVersionOf | |||||||||||||||||
| 識別子タイプ | DOI | |||||||||||||||||
| 関連識別子 | https://doi.org/10.31989/ffhd.v5i6.182 | |||||||||||||||||
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| 言語 | en | |||||||||||||||||
| 権利情報 | Copyright (c) 2015 Hiroko Hata, Masatoshi Yamaguchi, Hiroshi Sameshima, Tsuyomu Ikenoue, Junko Matsubara, Makoto Tsuboi, Takashi Tanaka | |||||||||||||||||
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| 出版タイプ | VoR | |||||||||||||||||
