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The HDAC Inhibitor, SAHA, Combined with Cisplatin Synergistically Induces Apoptosis in Alpha-fetoprotein-producing Hepatoid Adenocarcinoma Cells
http://hdl.handle.net/10458/0002000439
http://hdl.handle.net/10458/0002000439a3b750bd-5f9b-4418-80b0-0a346b7b08ed
| 名前 / ファイル | ライセンス | アクション |
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article (958 KB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||
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| 公開日 | 2024-01-19 | |||||||||||||
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| タイトル | The HDAC Inhibitor, SAHA, Combined with Cisplatin Synergistically Induces Apoptosis in Alpha-fetoprotein-producing Hepatoid Adenocarcinoma Cells | |||||||||||||
| 言語 | en | |||||||||||||
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| 言語 | eng | |||||||||||||
| キーワード | ||||||||||||||
| 言語 | en | |||||||||||||
| 主題Scheme | Other | |||||||||||||
| キーワード | HDAC inhibitor | |||||||||||||
| キーワード | ||||||||||||||
| 言語 | en | |||||||||||||
| 主題Scheme | Other | |||||||||||||
| キーワード | SAHA | |||||||||||||
| キーワード | ||||||||||||||
| 言語 | en | |||||||||||||
| 主題Scheme | Other | |||||||||||||
| キーワード | cisplatin | |||||||||||||
| キーワード | ||||||||||||||
| 言語 | en | |||||||||||||
| 主題Scheme | Other | |||||||||||||
| キーワード | apoptosis | |||||||||||||
| キーワード | ||||||||||||||
| 言語 | en | |||||||||||||
| 主題Scheme | Other | |||||||||||||
| キーワード | hepatoid adenocarcinoma | |||||||||||||
| 資源タイプ | ||||||||||||||
| 資源タイプ | journal article | |||||||||||||
| 著者 |
ミャ, ティン チュ チョウ
× ミャ, ティン チュ チョウ× 山口, 優也× チヨウジヨウフ, ナランツオツク
WEKO
26967
× 矢野, 公一× 高木, 秀明× 高橋, 伸育× ピュー, シン ウー× 佐藤, 克明× 菱川, 善隆 |
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| 抄録 | ||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||
| 内容記述 | Hepatoid adenocarcinoma (HAC) is a rare and aggressive gastrointestinal tract cancer that is characterized by hepatic differentiation and production of alpha-fetoprotein (AFP). Cisplatin is mainly used to treat HAC, but the efficacy is poor. Recently, the histone deacetylase inhibitor, suberoylanilide hydroxamic acid (SAHA), was approved as an anticancer agent. In this study, we investigated the anticancer effect of SAHA in combination with cisplatin in VAT-39 cells, a newly established HAC cell line. Cell viability and apoptosis were examined by MTT assay, flow cytometry and TUNEL assay. Expression of H3S10, cleaved caspase-3, Bax, and Bcl-2 were evaluated by immunohistochemistry and western blotting. AFP levels were examined in VAT-39 cells and culture medium. Combined treatment with cisplatin and SAHA efficiently inhibited cell proliferation and decreased cell viability. Apoptotic cells, but not necrotic cells, were significantly increased following the combined treatment, and an increase in the Bax/Bcl-2 ratio indicated that the combination of cisplatin and SAHA induced apoptosis through the mitochondrial pathway. VAT-39 cells treated with cisplatin and SAHA also partially lost their main characteristic of AFP production. We conclude that cisplatin and SAHA have a synergistic anticancer effect of inducing apoptosis, and that this combination treatment may be effective for HAC. | |||||||||||||
| 言語 | en | |||||||||||||
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| 内容記述タイプ | Other | |||||||||||||
| 内容記述 | Citation: Kyaw MTH, Yamaguchi Y, Choijookhuu N, Yano K, Takagi H, Takahashi N, Synn Oo P, Sato K, Hishikawa Y. The HDAC Inhibitor, SAHA, Combined with Cisplatin Synergistically Induces Apoptosis in Alpha-fetoprotein-producing Hepatoid Adenocarcinoma Cells. Acta Histochem Cytochem. 2019 Feb 28;52(1):1-8. doi: 10.1267/ahc.18044. Epub 2019 Feb 23. PMID: 30923410; PMCID: PMC6434315. |
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| 言語 | en | |||||||||||||
| 書誌情報 |
en : ACTA HISTOCHEMICA ET CYTOCHEMICA 巻 52, 号 1, p. 1-8, 発行日 2019-02-23 |
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| 出版者 | ||||||||||||||
| 出版者 | The Japan Society of Histochemistry and Cytochemistry | |||||||||||||
| 言語 | en | |||||||||||||
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| 収録物識別子タイプ | ISSN | |||||||||||||
| 収録物識別子 | 0044-5991 | |||||||||||||
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| 関連タイプ | isVersionOf | |||||||||||||
| 識別子タイプ | DOI | |||||||||||||
| 関連識別子 | https://doi.org/10.1267/ahc.18044 | |||||||||||||
| 権利 | ||||||||||||||
| 言語 | en | |||||||||||||
| 権利情報 | © 2019 The Japan Society of Histochemistry and Cytochemistry | |||||||||||||
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| 出版タイプ | VoR | |||||||||||||
