{"created":"2024-01-22T01:53:22.890123+00:00","id":2000439,"links":{},"metadata":{"_buckets":{"deposit":"a3b4009e-0cb7-4afa-80c1-e036b2d4c3c1"},"_deposit":{"created_by":11,"id":"2000439","owner":"11","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"2000439"},"status":"published"},"_oai":{"id":"oai:miyazaki-u.repo.nii.ac.jp:02000439","sets":["76","76:64"]},"author_link":["26963","26967","30111","30110","34847","27789","34794","34189","30105"],"control_number":"2000439","item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-02-23","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"8","bibliographicPageStart":"1","bibliographicVolumeNumber":"52","bibliographic_titles":[{"bibliographic_title":"ACTA HISTOCHEMICA ET CYTOCHEMICA","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Hepatoid adenocarcinoma (HAC) is a rare and aggressive gastrointestinal tract cancer that is characterized by hepatic differentiation and production of alpha-fetoprotein (AFP). Cisplatin is mainly used to treat HAC, but the efficacy is poor. Recently, the histone deacetylase inhibitor, suberoylanilide hydroxamic acid (SAHA), was approved as an anticancer agent. In this study, we investigated the anticancer effect of SAHA in combination with cisplatin in VAT-39 cells, a newly established HAC cell line. Cell viability and apoptosis were examined by MTT assay, flow cytometry and TUNEL assay. Expression of H3S10, cleaved caspase-3, Bax, and Bcl-2 were evaluated by immunohistochemistry and western blotting. AFP levels were examined in VAT-39 cells and culture medium. Combined treatment with cisplatin and SAHA efficiently inhibited cell proliferation and decreased cell viability. Apoptotic cells, but not necrotic cells, were significantly increased following the combined treatment, and an increase in the Bax/Bcl-2 ratio indicated that the combination of cisplatin and SAHA induced apoptosis through the mitochondrial pathway. VAT-39 cells treated with cisplatin and SAHA also partially lost their main characteristic of AFP production. We conclude that cisplatin and SAHA have a synergistic anticancer effect of inducing apoptosis, and that this combination treatment may be effective for HAC.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_description_6":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Citation:\nKyaw MTH, Yamaguchi Y, Choijookhuu N, Yano K, Takagi H, Takahashi N, Synn Oo P, Sato K, Hishikawa Y. The HDAC Inhibitor, SAHA, Combined with Cisplatin Synergistically Induces Apoptosis in Alpha-fetoprotein-producing Hepatoid Adenocarcinoma Cells. Acta Histochem Cytochem. 2019 Feb 28;52(1):1-8. doi: 10.1267/ahc.18044. Epub 2019 Feb 23. PMID: 30923410; PMCID: PMC6434315.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"The Japan Society of Histochemistry and Cytochemistry","subitem_publisher_language":"en"}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1267/ahc.18044","subitem_relation_type_select":"DOI"}}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2019 The Japan Society of Histochemistry and Cytochemistry","subitem_rights_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0044-5991","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Myat, Tin Htwe Kyaw","creatorNameLang":"en"},{"creatorName":"ミャ, ティン チュ チョウ","creatorNameLang":"ja"},{"creatorName":"ミャ, ティン チュ チョウ","creatorNameLang":"ja-Kana"}],"familyNames":[{"familyName":"Myat","familyNameLang":"en"},{"familyName":"ミャ","familyNameLang":"ja"},{"familyName":"ミャ","familyNameLang":"ja-Kana"}],"givenNames":[{"givenName":"Tin Htwe Kyaw","givenNameLang":"en"},{"givenName":"ティン チュ チョウ","givenNameLang":"ja"},{"givenName":"ティン チュ 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ナランツオツク","creatorNameLang":"ja-Kana"}],"familyNames":[{"familyName":"Choijookhuu","familyNameLang":"en"},{"familyName":"チヨウジヨウフ","familyNameLang":"ja"},{"familyName":"チヨウジヨウフ","familyNameLang":"ja-Kana"}],"givenNames":[{"givenName":"Narantsog","givenNameLang":"en"},{"givenName":"ナランツオツク","givenNameLang":"ja"},{"givenName":"ナランツオツク","givenNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"26967","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"90640962","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=90640962"}]},{"creatorNames":[{"creatorName":"矢野, 公一","creatorNameLang":"ja"},{"creatorName":"ヤノ, コウイチ","creatorNameLang":"ja-Kana"},{"creatorName":"Yano, 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ヒデアキ","creatorNameLang":"ja-Kana"}],"familyNames":[{"familyName":"Takagi","familyNameLang":"en"},{"familyName":"高木","familyNameLang":"ja"},{"familyName":"タカギ","familyNameLang":"ja-Kana"}],"givenNames":[{"givenName":"Hideaki","givenNameLang":"en"},{"givenName":"秀明","givenNameLang":"ja"},{"givenName":"ヒデアキ","givenNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"30105","nameIdentifierScheme":"WEKO"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"0000000106573887","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"https://isni.org/isni/0000000106573887"}],"affiliationNames":[{"affiliationName":"宮崎大学","affiliationNameLang":"ja"},{"affiliationName":"University of Miyazaki","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"高橋, 伸育","creatorNameLang":"ja"},{"creatorName":"タカハシ, ノブヤス","creatorNameLang":"ja-Kana"},{"creatorName":"Takahashi, Nobuyasu","creatorNameLang":"en"}],"familyNames":[{"familyName":"高橋","familyNameLang":"ja"},{"familyName":"タカハシ","familyNameLang":"ja-Kana"},{"familyName":"Takahashi","familyNameLang":"en"}],"givenNames":[{"givenName":"伸育","givenNameLang":"ja"},{"givenName":"ノブヤス","givenNameLang":"ja-Kana"},{"givenName":"Nobuyasu","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"34847","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"20404436","nameIdentifierScheme":"e-Rad_Researcher","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=20404436"}]},{"creatorNames":[{"creatorName":"Phyu, Synn  Oo","creatorNameLang":"en"},{"creatorName":"ピュー, シン ウー","creatorNameLang":"ja"},{"creatorName":"ピュー, シン ウー","creatorNameLang":"ja-Kana"}],"familyNames":[{"familyName":"Phyu","familyNameLang":"en"},{"familyName":"ピュー","familyNameLang":"ja"},{"familyName":"ピュー","familyNameLang":"ja-Kana"}],"givenNames":[{"givenName":"Synn  Oo","givenNameLang":"en"},{"givenName":"シン 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善隆","creatorNameLang":"ja"},{"creatorName":"ヒシカワ, ヨシタカ","creatorNameLang":"ja-Kana"},{"creatorName":"Hishikawa, Yoshitaka","creatorNameLang":"en"}],"familyNames":[{"familyName":"菱川","familyNameLang":"ja"},{"familyName":"ヒシカワ","familyNameLang":"ja-Kana"},{"familyName":"Hishikawa","familyNameLang":"en"}],"givenNames":[{"givenName":"善隆","givenNameLang":"ja"},{"givenName":"ヨシタカ","givenNameLang":"ja-Kana"},{"givenName":"Yoshitaka","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"34189","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"60304276","nameIdentifierScheme":"e-Rad_Researcher","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=60304276"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2024-01-22"}],"filename":"article.pdf","filesize":[{"value":"958 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adenocarcinoma","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"The HDAC Inhibitor, SAHA, Combined with Cisplatin Synergistically Induces Apoptosis in Alpha-fetoprotein-producing Hepatoid Adenocarcinoma Cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"The HDAC Inhibitor, SAHA, Combined with Cisplatin Synergistically Induces Apoptosis in Alpha-fetoprotein-producing Hepatoid Adenocarcinoma Cells","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"11","path":["76","64"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2024-01-19"},"publish_date":"2024-01-19","publish_status":"0","recid":"2000439","relation_version_is_last":true,"title":["The HDAC Inhibitor, SAHA, Combined with Cisplatin Synergistically Induces Apoptosis in Alpha-fetoprotein-producing Hepatoid Adenocarcinoma Cells"],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2025-01-17T02:46:33.004385+00:00"}