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Spatiotemporal expression of HMGB2 regulates cell proliferation and hepatocyte size during liver regeneration
http://hdl.handle.net/10458/0002000301
http://hdl.handle.net/10458/000200030107c3b718-dce8-4a9c-8646-1486c8a7cc05
| 名前 / ファイル | ライセンス | アクション |
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本文 (2.8 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||
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| 公開日 | 2023-11-07 | |||||||||||||
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| タイトル | Spatiotemporal expression of HMGB2 regulates cell proliferation and hepatocyte size during liver regeneration | |||||||||||||
| 言語 | en | |||||||||||||
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| 言語 | eng | |||||||||||||
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| 資源タイプ | journal article | |||||||||||||
| 著者 |
矢野, 公一
× 矢野, 公一× チヨウジヨウフ, ナランツオツク
WEKO
26967
× 池ノ上, 実× Fidya× 深谷, 知宏× 佐藤, 克明× 李, 徳哲× 谷口, 昇× 帖佐, 悦男× 七島, 篤志× 菱川, 善隆 |
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| 抄録 | ||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||
| 内容記述 | Liver regeneration is an extraordinarily complex process involving a variety of factors; however, the role of chromatin protein in hepatocyte proliferation is largely unknown. In this study, we investigated the functional role of high-mobility group box 2 (HMGB2), a chromatin protein in liver regeneration using wild-type and HMGB2-knockout (KO) mice. Liver tissues were sampled after 70% partial hepatectomy (PHx), and analyzed by immunohistochemistry, western blotting and flow cytometry using various markers of cell proliferation. In WT mice, hepatocyte proliferation was strongly correlated with the spatiotemporal expression of HMGB2; however, cell proliferation was significantly delayed in hepatocytes of HMGB2-KO mice. Quantitative PCR demonstrated that cyclin D1 and cyclin B1 mRNAs were significantly decreased in HMGB2-KO mice livers. Interestingly, hepatocyte size was significantly larger in HMGB2-KO mice at 36–72 h after PHx, and these results suggest that hepatocyte hypertrophy appeared in parallel with delayed cell proliferation. In vitro experiments demonstrated that cell proliferation was significantly decreased in HMGB2-KO cells. A significant delay in cell proliferation was also found in HMGB2-siRNA transfected cells. In summary, spatiotemporal expression of HMGB2 is important for regulation of hepatocyte proliferation and cell size during liver regeneration. | |||||||||||||
| 言語 | en | |||||||||||||
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| 内容記述タイプ | Other | |||||||||||||
| 内容記述 | Citation: Yano K, Choijookhuu N, Ikenoue M, Fidya, Fukaya T, Sato K, Lee D, Taniguchi N, Chosa E, Nanashima A, Hishikawa Y. Spatiotemporal expression of HMGB2 regulates cell proliferation and hepatocyte size during liver regeneration. Sci Rep. 2022 Jul 13;12(1):11962. doi: 10.1038/s41598-022-16258-4. PMID: 35831365; PMCID: PMC9279446. |
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| 言語 | en | |||||||||||||
| 書誌情報 |
en : Scientific Reports 巻 12, p. 11962 (2022), 発行日 2022-07-13 |
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| 出版者 | ||||||||||||||
| 出版者 | Springer Nature | |||||||||||||
| 言語 | en | |||||||||||||
| DOI | ||||||||||||||
| 関連タイプ | isVersionOf | |||||||||||||
| 識別子タイプ | DOI | |||||||||||||
| 関連識別子 | https://doi.org/10.1038/s41598-022-16258-4 | |||||||||||||
| 権利 | ||||||||||||||
| 言語 | en | |||||||||||||
| 権利情報 | © Te Author(s) 2022 | |||||||||||||
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| 出版タイプ | VoR | |||||||||||||
