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Characterization of bovine interleukin-2 stably expressed in HEK-293 cells
http://hdl.handle.net/10458/0002000184
http://hdl.handle.net/10458/000200018466383c01-4e98-4e1b-bb91-443917532478
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
本文 (2.2 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||
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| 公開日 | 2023-10-30 | |||||||
| タイトル | ||||||||
| タイトル | Characterization of bovine interleukin-2 stably expressed in HEK-293 cells | |||||||
| 言語 | en | |||||||
| 言語 | ||||||||
| 言語 | eng | |||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| キーワード | bovine interleukin-2 | |||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| キーワード | bovine interleukin-2 monoclonal antibody | |||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| キーワード | stable expression | |||||||
| 資源タイプ | ||||||||
| 資源タイプ | journal article | |||||||
| 著者 |
三苫, 修也
× 三苫, 修也× El-Khaiat Heba M.
× 宇都, 倫史× 佐藤, 克明× 関口, 敏× 乗峰, 潤三 |
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| 抄録 | ||||||||
| 内容記述タイプ | Abstract | |||||||
| 内容記述 | Interleukin 2 (IL-2) is a pleotropic cytokine and well-known as a T cell growth factor in immunology. It is now known to exert both immunostimulatory and immunosuppressive effects, optimizing immunological microenvironments for effector and regulatory T cell responses. The immunomodulatory role of IL-2 is critical for deciding whether or not T cell responses against specific antigens result in protection. We have established a mammalian cell line (HEK-293) stably expressing bovine IL-2 (boIL-2) (designated as HEK-293/boIL-2), using the piggyBac transposon system. The concentration of recombinant bovine IL-2 (rboIL-2) in the culture supernatant of HEK-293/boIL-2 reached 100 ng/ml on day 7 and showed similar proliferative activity to recombinant human IL-2 (rhuIL-2) for bovine peripheral mononuclear blood cells. Although rhuIL-2 has been often used to activate bovine T cells, our results indicate that characteristics of the T cell activation through rboIL-2 and huIL-2 appear slightly but significantly different. Interestingly, the rboIL-2/anti-boIL-2 monoclonal antibody (C5) (rboIL-2/C5) complex strongly induced proliferation of bovine NKp46+cells, natural killer (NK) cells, in vitro. This indicates that the rboIL-2/C5 complex could function as an IL-2 agonist specifically to increase the NK cell population, which in turn could enhance the activity of NK cells leading to protective immunity. | |||||||
| 言語 | en | |||||||
| 内容記述 | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | Citation: Mitoma S, El-Khaiat HM, Uto T, Sato K, Sekiguchi S, Norimine J. Characterization of bovine interleukin-2 stably expressed in HEK-293 cells. J Vet Med Sci. 2021 Jan 21;83(1):134-141. doi: 10.1292/jvms.20-0423. Epub 2020 Nov 11. PMID: 33177289; PMCID: PMC7870392. |
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| 言語 | en | |||||||
| 書誌情報 |
en : Journal of Veterinary Medical Science 巻 83, 号 1, p. 134-141, 発行日 2021 |
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| 出版者 | ||||||||
| 出版者 | Japanese Society of Veterinary Science | |||||||
| 言語 | en | |||||||
| ISSN | ||||||||
| 収録物識別子タイプ | ISSN | |||||||
| 収録物識別子 | 0916-7250 | |||||||
| DOI | ||||||||
| 関連タイプ | isVersionOf | |||||||
| 識別子タイプ | DOI | |||||||
| 関連識別子 | https://doi.org/10.1292/jvms.20-0423 | |||||||
| 権利 | ||||||||
| 言語 | en | |||||||
| 権利情報 | © 2021 by the Japanese Society of Veterinary Science | |||||||
| 著者版フラグ | ||||||||
| 出版タイプ | VoR | |||||||
