{"created":"2023-10-30T05:34:18.723197+00:00","id":2000184,"links":{},"metadata":{"_buckets":{"deposit":"4d7242ba-a4ba-4da7-9d96-866b355e0ebc"},"_deposit":{"created_by":9,"id":"2000184","owner":"9","owners":[9],"pid":{"revision_id":0,"type":"depid","value":"2000184"},"status":"published"},"_oai":{"id":"oai:miyazaki-u.repo.nii.ac.jp:02000184","sets":["76","76:64"]},"author_link":["24383","30111","30185","1751","34689"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"141","bibliographicPageStart":"134","bibliographicVolumeNumber":"83","bibliographic_titles":[{"bibliographic_title":"Journal of Veterinary Medical Science","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Interleukin 2 (IL-2) is a pleotropic cytokine and well-known as a T cell growth factor in immunology. It is now known to exert both immunostimulatory and immunosuppressive effects, optimizing immunological microenvironments for effector and regulatory T cell responses. The immunomodulatory role of IL-2 is critical for deciding whether or not T cell responses against specific antigens result in protection. We have established a mammalian cell line (HEK-293) stably expressing bovine IL-2 (boIL-2) (designated as HEK-293/boIL-2), using the piggyBac transposon system. The concentration of recombinant bovine IL-2 (rboIL-2) in the culture supernatant of HEK-293/boIL-2 reached 100 ng/ml on day 7 and showed similar proliferative activity to recombinant human IL-2 (rhuIL-2) for bovine peripheral mononuclear blood cells. Although rhuIL-2 has been often used to activate bovine T cells, our results indicate that characteristics of the T cell activation through rboIL-2 and huIL-2 appear slightly but significantly different. Interestingly, the rboIL-2/anti-boIL-2 monoclonal antibody (C5) (rboIL-2/C5) complex strongly induced proliferation of bovine NKp46+cells, natural killer (NK) cells, in vitro. This indicates that the rboIL-2/C5 complex could function as an IL-2 agonist specifically to increase the NK cell population, which in turn could enhance the activity of NK cells leading to protective immunity.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_description_6":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Citation:\nMitoma S, El-Khaiat HM, Uto T, Sato K, Sekiguchi S, Norimine J. Characterization of bovine interleukin-2 stably expressed in HEK-293 cells. J Vet Med Sci. 2021 Jan 21;83(1):134-141. doi: 10.1292/jvms.20-0423. Epub 2020 Nov 11. PMID: 33177289; PMCID: PMC7870392.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Japanese Society of Veterinary Science","subitem_publisher_language":"en"}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1292/jvms.20-0423","subitem_relation_type_select":"DOI"}}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2021 by the Japanese Society of Veterinary Science","subitem_rights_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0916-7250","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{}],"affiliationNames":[{},{}]}],"creatorNames":[{"creatorName":"三苫, 修也","creatorNameLang":"ja"},{"creatorName":"ミトマ, シュウヤ","creatorNameLang":"ja-Kana"},{"creatorName":"Mitoma, Shuya","creatorNameLang":"en"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{"nameIdentifier":"34689","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"El-Khaiat Heba M.","creatorNameLang":"en"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{}],"affiliationNames":[{},{}]}],"creatorNames":[{"creatorName":"Uto, Tomofumi","creatorNameLang":"en"},{"creatorName":"宇都, 倫史","creatorNameLang":"ja"},{"creatorName":"ウト, トモフミ","creatorNameLang":"ja-Kana"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{"nameIdentifier":"30185","nameIdentifierScheme":"WEKO"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{}],"affiliationNames":[{},{}]}],"creatorNames":[{"creatorName":"Sato, Katsuaki","creatorNameLang":"en"},{"creatorName":"佐藤, 克明","creatorNameLang":"ja"},{"creatorName":"サトウ, カツアキ","creatorNameLang":"ja-Kana"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{"nameIdentifier":"30111","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"40301147","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=40301147"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{}],"affiliationNames":[{},{}]}],"creatorNames":[{"creatorName":"Sekiguchi, Satoshi","creatorNameLang":"en"},{"creatorName":"関口, 敏","creatorNameLang":"ja"},{"creatorName":"セキグチ, サトシ","creatorNameLang":"ja-Kana"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{"nameIdentifier":"24383","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"10462780","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=10462780"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{}],"affiliationNames":[{},{}]}],"creatorNames":[{"creatorName":"Norimine, Junzo","creatorNameLang":"en"},{"creatorName":"乗峰, 潤三","creatorNameLang":"ja"},{"creatorName":"ノリミネ, ジユンゾウ","creatorNameLang":"ja-Kana"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{"nameIdentifier":"1751","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-10-30"}],"filename":"83_20-0423.pdf","filesize":[{"value":"2.2 MB"}],"format":"application/pdf","licensetype":"license_5","url":{"label":"本文","objectType":"fulltext","url":"https://miyazaki-u.repo.nii.ac.jp/record/2000184/files/83_20-0423.pdf"},"version_id":"cbc81549-70ea-4fd0-a2ef-441ff92c53b2"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"bovine interleukin-2","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"bovine interleukin-2 monoclonal antibody","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"stable expression","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Characterization of bovine interleukin-2 stably expressed in HEK-293 cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Characterization of bovine interleukin-2 stably expressed in HEK-293 cells","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"9","path":["76","64"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-10-30"},"publish_date":"2023-10-30","publish_status":"0","recid":"2000184","relation_version_is_last":true,"title":["Characterization of bovine interleukin-2 stably expressed in HEK-293 cells"],"weko_creator_id":"9","weko_shared_id":-1},"updated":"2024-07-16T08:20:57.263642+00:00"}