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Photodynamic Therapy Using a Novel Phosphorus Tetraphenylporphyrin Induces an Anticancer Effect via Bax/Bcl-xL-related Mitochondrial Apoptosis in Biliary Cancer Cells
http://hdl.handle.net/10458/0002000154
http://hdl.handle.net/10458/0002000154a1d98cd7-0ab3-475a-9b39-2514a81ea412
| 名前 / ファイル | ライセンス | アクション |
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本文 (5.5 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||
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| 公開日 | 2023-10-26 | |||||||||||||
| タイトル | ||||||||||||||
| タイトル | Photodynamic Therapy Using a Novel Phosphorus Tetraphenylporphyrin Induces an Anticancer Effect via Bax/Bcl-xL-related Mitochondrial Apoptosis in Biliary Cancer Cells | |||||||||||||
| 言語 | en | |||||||||||||
| 言語 | ||||||||||||||
| 言語 | eng | |||||||||||||
| キーワード | ||||||||||||||
| 言語 | en | |||||||||||||
| 主題Scheme | Other | |||||||||||||
| キーワード | photodynamic therapy | |||||||||||||
| キーワード | ||||||||||||||
| 言語 | en | |||||||||||||
| 主題Scheme | Other | |||||||||||||
| キーワード | phosphorus tetraphenylporphyrin | |||||||||||||
| キーワード | ||||||||||||||
| 言語 | en | |||||||||||||
| 主題Scheme | Other | |||||||||||||
| キーワード | mitochondrial apoptosis | |||||||||||||
| キーワード | ||||||||||||||
| 言語 | en | |||||||||||||
| 主題Scheme | Other | |||||||||||||
| キーワード | Bax/Bcl-xL | |||||||||||||
| キーワード | ||||||||||||||
| 言語 | en | |||||||||||||
| 主題Scheme | Other | |||||||||||||
| キーワード | anticancer effect | |||||||||||||
| 資源タイプ | ||||||||||||||
| 資源タイプ | journal article | |||||||||||||
| 著者 |
グエン, マイ ナット ヒュン
× グエン, マイ ナット ヒュン× 山口, 優也× チヨウジヨウフ, ナランツオツク
WEKO
26967
× 松本, 仁× 七島, 篤志× 高木, 秀明× 佐藤, 克明× Tuan, Le Quoc× 菱川, 善隆 |
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| 抄録 | ||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||
| 内容記述 | Photodynamic therapy (PDT) uses photosensitizer activation by light of a specific wavelength, and is a promising treatment for various cancers; however, the detailed mechanism of PDT remains unclear. Therefore, we investigated the anticancer effect of PDT using a novel phosphorus tetraphenylporphyrin (Ptpp) in combination with light emitting diodes (Ptpp-PDT) in the NOZ human biliary cancer cell line. Cell viability and apoptosis were examined by MTT assay, flow cytometry and TUNEL assay for 24 hr after Ptpp-PDT. MitoTracker and JC-1 were used as markers of mitochondrial localization and membrane potential. The levels of mitochondrial oxidative phosphorylation (OXPHOS) complexes, Bcl-2 family proteins, cytochrome c and cleaved caspase-3 were examined by western blotting and immunohistochemistry. The results revealed that Ptpp localized to mitochondria, and that Ptpp-PDT efficiently decreased cell viability in a dose- and time-dependent manner. JC-1 and OXPHOS complexes decreased, but apoptotic cells increased from 6 to 24 hr after Ptpp-PDT. A decrease in Bcl-xL and increases in Bax, cytochrome c and cleaved caspase-3 were also found from 6 to 24 hr after Ptpp-PDT. Based on these results, we conclude that Ptpp-PDT induces anticancer effects via the mitochondrial apoptotic pathway by altering the Bax/Bcl-xL ratio, and could be an effective treatment for human biliary cancer. | |||||||||||||
| 言語 | en | |||||||||||||
| 内容記述 | ||||||||||||||
| 内容記述タイプ | Other | |||||||||||||
| 内容記述 | Citation: Nguyen Nhat Huynh Mai, Yuya Yamaguchi, Narantsog Choijookhuu, Jin Matsumoto, Atsushi Nanashima, Hideaki Takagi, Katsuaki Sato, Le Quoc Tuan, Yoshitaka Hishikawa, Photodynamic Therapy Using a Novel Phosphorus Tetraphenylporphyrin Induces an Anticancer Effect via Bax/Bcl-xL-related Mitochondrial Apoptosis in Biliary Cancer Cells, ACTA HISTOCHEMICA ET CYTOCHEMICA, 2020, Volume 53, Issue 4, Pages 61-72, Released on J-STAGE August 26, 2020, Advance online publication July 04, 2020, Online ISSN 1347-5800, Print ISSN 0044-5991, https://doi.org/10.1267/ahc.20-00002 |
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| 言語 | en | |||||||||||||
| 書誌情報 |
en : ACTA HISTOCHEMICA ET CYTOCHEMICA 巻 53, 号 4, p. 61-72, 発行日 2020-08-26 |
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| 出版者 | ||||||||||||||
| 出版者 | Japan Society of Histochemistry and Cytochemistry | |||||||||||||
| 言語 | en | |||||||||||||
| ISSN | ||||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||||
| 収録物識別子 | 0044-5991 | |||||||||||||
| DOI | ||||||||||||||
| 関連タイプ | isVersionOf | |||||||||||||
| 識別子タイプ | DOI | |||||||||||||
| 関連識別子 | https://doi.org/10.1267/ahc.20-00002 | |||||||||||||
| 権利 | ||||||||||||||
| 言語 | en | |||||||||||||
| 権利情報 | © 2020 The Japan Society of Histochemistry and Cytochemistry | |||||||||||||
| 著者版フラグ | ||||||||||||||
| 出版タイプ | VoR | |||||||||||||
