{"created":"2023-10-26T05:13:59.561372+00:00","id":2000154,"links":{},"metadata":{"_buckets":{"deposit":"abc82992-328d-43ec-82c8-14da69a41bc4"},"_deposit":{"created_by":9,"id":"2000154","owner":"9","owners":[9],"pid":{"revision_id":0,"type":"depid","value":"2000154"},"status":"published"},"_oai":{"id":"oai:miyazaki-u.repo.nii.ac.jp:02000154","sets":["76","76:64"]},"author_link":["26963","26967","30107","6572","30111","22789","34189","33886","30105"],"control_number":"2000154","item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-08-26","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"72","bibliographicPageStart":"61","bibliographicVolumeNumber":"53","bibliographic_titles":[{"bibliographic_title":"ACTA HISTOCHEMICA ET CYTOCHEMICA","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Photodynamic therapy (PDT) uses photosensitizer activation by light of a specific wavelength, and is a promising treatment for various cancers; however, the detailed mechanism of PDT remains unclear. Therefore, we investigated the anticancer effect of PDT using a novel phosphorus tetraphenylporphyrin (Ptpp) in combination with light emitting diodes (Ptpp-PDT) in the NOZ human biliary cancer cell line. Cell viability and apoptosis were examined by MTT assay, flow cytometry and TUNEL assay for 24 hr after Ptpp-PDT. MitoTracker and JC-1 were used as markers of mitochondrial localization and membrane potential. The levels of mitochondrial oxidative phosphorylation (OXPHOS) complexes, Bcl-2 family proteins, cytochrome c and cleaved caspase-3 were examined by western blotting and immunohistochemistry. The results revealed that Ptpp localized to mitochondria, and that Ptpp-PDT efficiently decreased cell viability in a dose- and time-dependent manner. JC-1 and OXPHOS complexes decreased, but apoptotic cells increased from 6 to 24 hr after Ptpp-PDT. A decrease in Bcl-xL and increases in Bax, cytochrome c and cleaved caspase-3 were also found from 6 to 24 hr after Ptpp-PDT. Based on these results, we conclude that Ptpp-PDT induces anticancer effects via the mitochondrial apoptotic pathway by altering the Bax/Bcl-xL ratio, and could be an effective treatment for human biliary cancer.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_description_6":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Citation:\nNguyen Nhat Huynh Mai, Yuya Yamaguchi, Narantsog Choijookhuu, Jin Matsumoto, Atsushi Nanashima, Hideaki Takagi, Katsuaki Sato, Le Quoc Tuan, Yoshitaka Hishikawa, Photodynamic Therapy Using a Novel Phosphorus Tetraphenylporphyrin Induces an Anticancer Effect via Bax/Bcl-xL-related Mitochondrial Apoptosis in Biliary Cancer Cells, ACTA HISTOCHEMICA ET CYTOCHEMICA, 2020, Volume 53, Issue 4, Pages 61-72, Released on J-STAGE August 26, 2020, Advance online publication July 04, 2020, Online ISSN 1347-5800, Print ISSN 0044-5991, https://doi.org/10.1267/ahc.20-00002","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Japan Society of Histochemistry and Cytochemistry","subitem_publisher_language":"en"}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1267/ahc.20-00002","subitem_relation_type_select":"DOI"}}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2020 The Japan Society of Histochemistry and Cytochemistry","subitem_rights_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0044-5991","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Nguyen, Mai Nhat Huynh","creatorNameLang":"en"},{"creatorName":"グエン, マイ ナット ヒュン","creatorNameLang":"ja"},{"creatorName":"グエン, マイ ナット ヒュン","creatorNameLang":"ja-Kana"}],"familyNames":[{"familyName":"Nguyen","familyNameLang":"en"},{"familyName":"グエン","familyNameLang":"ja"},{"familyName":"グエン","familyNameLang":"ja-Kana"}],"givenNames":[{"givenName":"Mai Nhat Huynh","givenNameLang":"en"},{"givenName":"マイ ナット ヒュン","givenNameLang":"ja"},{"givenName":"マイ ナット 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仁","creatorNameLang":"ja"},{"creatorName":"マツモト, ジン","creatorNameLang":"ja-Kana"},{"creatorName":"Matsumoto, Jin","creatorNameLang":"en"}],"familyNames":[{"familyName":"松本","familyNameLang":"ja"},{"familyName":"マツモト","familyNameLang":"ja-Kana"},{"familyName":"Matsumoto","familyNameLang":"en"}],"givenNames":[{"givenName":"仁","givenNameLang":"ja"},{"givenName":"ジン","givenNameLang":"ja-Kana"},{"givenName":"Jin","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"6572","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"90363572","nameIdentifierScheme":"e-Rad_Researcher","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=90363572"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"0000000106573887","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"https://isni.org/isni/0000000106573887"}],"affiliationNames":[{"affiliationName":"宮崎大学","affiliationNameLang":"ja"},{"affiliationName":"University of 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effect","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Photodynamic Therapy Using a Novel Phosphorus Tetraphenylporphyrin Induces an Anticancer Effect via Bax/Bcl-xL-related Mitochondrial Apoptosis in Biliary Cancer Cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Photodynamic Therapy Using a Novel Phosphorus Tetraphenylporphyrin Induces an Anticancer Effect via Bax/Bcl-xL-related Mitochondrial Apoptosis in Biliary Cancer Cells","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"9","path":["76","64"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-10-26"},"publish_date":"2023-10-26","publish_status":"0","recid":"2000154","relation_version_is_last":true,"title":["Photodynamic Therapy Using a Novel Phosphorus Tetraphenylporphyrin Induces an Anticancer Effect via Bax/Bcl-xL-related Mitochondrial Apoptosis in Biliary Cancer Cells"],"weko_creator_id":"9","weko_shared_id":-1},"updated":"2025-01-17T02:46:35.993278+00:00"}