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Expression of Adrenomedullin and Proadrenomedullin N-Terminal 20 Peptide in PC12 Cells after Exposure to Nerve Growth Factor
http://hdl.handle.net/10458/1512
http://hdl.handle.net/10458/15120dead7ba-9f0d-41b2-a521-ff5a131ee417
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
Neuroscience 125(4) p.73-980,2004.pdf (1.4 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2008-04-09 | |||||
| タイトル | ||||||
| タイトル | Expression of Adrenomedullin and Proadrenomedullin N-Terminal 20 Peptide in PC12 Cells after Exposure to Nerve Growth Factor | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | Adrenomedullin, PAMP, Nerve growth factor, Neuronal differentiation, Expression, PC12 cells | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| 著者 |
小林, 英幸
× 小林, 英幸× 柳田, 俊彦× 横尾, 宏毅× 和田, 明彦× Itoh, Satoru× 横尾, 宏毅× Wada, Akihiko |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Adrenomedullin （AM） and proadrenomedullin N-terminal 20 peptide （PAMP） are multi-functional peptides derived from the same precursor, proadrenomedullin. We have studied the regulatory mechanism of expression of these peptides during neuronal differentiation of rat pheochromocytoma PC12 cells by nerve growth factor （NGF）. The cellular levels of the peptides increased slightly, and then progressively decreased below the control by NGF. Immunoreactive （ir）- AM in the medium was transiently increased by NGF. Cytochemical staining showed that ir-AM and ir-PAMP were abundantly present in cytoplasm in the undifferentiated cells, and were decreased during culture with NGF. There was no preferential localization of ir-AM or ir-PAMP in neurites in comparison with in cytoplasm in the differentiated cells. Northern blot analysis showed that mRNA encoding these peptides, as detected as a band of 1.6 kb, increased more than 3-fold at 1 hour after the addition of NGF and then progressively decreased to 1/5 of the control during 72 hours. Degradation rate of the mRNA was slowed by NGF even when mRNA level is decreased after 72 hours of NGF treatment. The transcription rate of their gene increased transiently and then decreased by the long-term treatment with NGF. These results demonstrate that expression of AM and PAMP is regulated by NGF along with time-dependent differentiation: AM gene transcription is transiently activated by NGF, whereas was suppressed during neuronal differentiation of the cells. |
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| 言語 | en | |||||
| 書誌情報 |
en : Neuroscience 巻 125, 号 4, p. 973-980, 発行日 2004 |
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| 出版者 | ||||||
| 出版者 | Elsevier | |||||
| 言語 | en | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 03064522 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA0075489X | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | http://www.elsevier.com/wps/find/journaldescription.cws_home/468/description | |||||
| 関連名称 | http://www.elsevier.com/wps/find/journaldescription.cws_home/468/description | |||||
| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
