{"created":"2023-05-15T09:57:46.315823+00:00","id":1614,"links":{},"metadata":{"_buckets":{"deposit":"59313dc5-843e-4659-bb4f-1fedbf196d5b"},"_deposit":{"created_by":5,"id":"1614","owner":"5","owners":[5],"pid":{"revision_id":0,"type":"depid","value":"1614"},"status":"published"},"_oai":{"id":"oai:miyazaki-u.repo.nii.ac.jp:00001614","sets":["72","72:30"]},"author_link":["9724","7904","7906","7905","7909","7907"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2004","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"980","bibliographicPageStart":"973","bibliographicVolumeNumber":"125","bibliographic_titles":[{"bibliographic_title":"Neuroscience","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Adrenomedullin （AM） and proadrenomedullin N-terminal 20 peptide （PAMP）\nare multi-functional peptides derived from the same precursor, proadrenomedullin.\nWe have studied the regulatory mechanism of expression of these peptides during\nneuronal differentiation of rat pheochromocytoma PC12 cells by nerve growth factor\n（NGF）. The cellular levels of the peptides increased slightly, and then progressively\ndecreased below the control by NGF. Immunoreactive （ir）- AM in the medium was\ntransiently increased by NGF. Cytochemical staining showed that ir-AM and\nir-PAMP were abundantly present in cytoplasm in the undifferentiated cells, and were\ndecreased during culture with NGF. There was no preferential localization of ir-AM\nor ir-PAMP in neurites in comparison with in cytoplasm in the differentiated cells.\nNorthern blot analysis showed that mRNA encoding these peptides, as detected as a\nband of 1.6 kb, increased more than 3-fold at 1 hour after the addition of NGF and then\nprogressively decreased to 1/5 of the control during 72 hours. Degradation rate of the\nmRNA was slowed by NGF even when mRNA level is decreased after 72 hours of NGF\ntreatment. The transcription rate of their gene increased transiently and then decreased\nby the long-term treatment with NGF. These results demonstrate that expression of\nAM and PAMP is regulated by NGF along with time-dependent differentiation: AM\ngene transcription is transiently activated by NGF, whereas was suppressed during\nneuronal differentiation of the cells.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier","subitem_publisher_language":"en"}]},"item_10001_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"http://www.elsevier.com/wps/find/journaldescription.cws_home/468/description"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.elsevier.com/wps/find/journaldescription.cws_home/468/description","subitem_relation_type_select":"URI"}}]},"item_10001_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA0075489X","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03064522","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"小林, 英幸","creatorNameLang":"ja"},{"creatorName":"コバヤシ, ヒデユキ","creatorNameLang":"ja-Kana"},{"creatorName":"Kobayashi, Hideyuki","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"柳田, 俊彦","creatorNameLang":"ja"},{"creatorName":"ヤナギタ, トシヒコ","creatorNameLang":"ja-Kana"},{"creatorName":"Yanagita, Toshihiko","creatorNameLang":"en"}],"nameIdentifiers":[{},{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"横尾, 宏毅","creatorNameLang":"ja"},{"creatorName":"ヨコオ, ヒロキ","creatorNameLang":"ja-Kana"},{"creatorName":"Yokoo, Hiroki","creatorNameLang":"en"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"和田, 明彦","creatorNameLang":"ja"},{"creatorName":"ワダ, アキヒコ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Itoh, Satoru","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"横尾, 宏毅","creatorNameLang":"ja"},{"creatorName":"ヨコオ, ヒロキ","creatorNameLang":"ja-Kana"},{"creatorName":"Yokoo, Hiroki","creatorNameLang":"en"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Wada, Akihiko","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-06-21"}],"displaytype":"detail","filename":"neuroscience_125-4-p.73-980_2004.pdf","filesize":[{"value":"1.4 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Neuroscience 125(4) p.73-980,2004.pdf","url":"https://miyazaki-u.repo.nii.ac.jp/record/1614/files/neuroscience_125-4-p.73-980_2004.pdf"},"version_id":"66c2b81b-3818-4ab2-943e-ea455b679ede"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Adrenomedullin, PAMP, Nerve growth factor, Neuronal differentiation, Expression, PC12 cells","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Expression of Adrenomedullin and Proadrenomedullin N-Terminal 20 Peptide in PC12 Cells after Exposure to Nerve Growth Factor","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Expression of Adrenomedullin and Proadrenomedullin N-Terminal 20 Peptide in PC12 Cells after Exposure to Nerve Growth Factor","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"5","path":["72","30"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2008-04-09"},"publish_date":"2008-04-09","publish_status":"0","recid":"1614","relation_version_is_last":true,"title":["Expression of Adrenomedullin and Proadrenomedullin N-Terminal 20 Peptide in PC12 Cells after Exposure to Nerve Growth Factor"],"weko_creator_id":"5","weko_shared_id":2},"updated":"2024-01-30T04:26:52.961837+00:00"}