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  1. 農学部
  1. 農学部
  2. 学術雑誌掲載論文 (農学部)

Characterization of functional P2X1 receptors in mouse megakaryocytes

http://hdl.handle.net/10458/1127
http://hdl.handle.net/10458/1127
8e9c0cf5-2d1d-407a-8621-26bdab9202e5
名前 / ファイル ライセンス アクション
TP-2007-Ikeda.pdf TP-2007-Ikeda.pdf (695.0 kB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2008-01-29
タイトル
タイトル Characterization of functional P2X1 receptors in mouse megakaryocytes
言語 en
言語
言語 eng
キーワード
言語 en
主題Scheme Other
キーワード megakaryocytes, platelets, ATP, P2X1, patch-clamp
資源タイプ
資源タイプ journal article
著者 池田, 正浩

× 池田, 正浩

WEKO 1081
e-Rad_Researcher 60281218

ja 池田, 正浩

ja-Kana イケダ, マサヒロ

en Ikeda, Masahiro


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内容記述タイプ Abstract
内容記述 Introduction: Although accumulating evidence within the past five years strongly supports the importance of platelet P2X1 receptors in hemostasis and thrombosis, P2X1 receptors of platelet and /or its progenitor cell, megakaryocyte, have not been fully characterized. The aim of this study was to electrophysiologically and pharamacologically characterize the functional P2X1 receptors on mouse
megakaryocytes.
Materials and Methods: The currents in response to nucleotides were examined using the patch-clamp whole-cell recording.
Results: The agonist profile of megakaryocyte P2X1 receptors was ATP >α,β-methyleneATP > β,γ-methyleneATP. The P2X1 receptors exhibited substantial monovalent as well as divalent cation permeability and the ratios of Na+ to Cs+ and Ca2+ to Cs+ permeability were 1 and 2.5, respectively.
P2X receptor antagonists except suramin significantly inhibited the P2X1 responses with an IC50 values of 0.4 μM for pyridoxal-phosphate-6-azophenyl-2',4'-disulphonate (PPADS), 0.3 μM for 2',3'-O-(2,4,6-trinitophenyl)- adenosine 5'-triphosphate (TNP-ATP), 20 μM for reactive blue 2 (RB2),
or 160 μM for 8,8'-(carbonylbis(imino-3,1-phenylene carbonylimino)bis(1,3,5-naphthalenetrisulfonic acid) (NF023), respectively. Suramin had no significant effect on the P2X1 responses. In rat
megakaryocytes, suramin similarly had no significant effect on the P2X1 responses, but abolished the P2Y receptor-mediated responses, indicating that the suramin was active under present experimental
condition.
Conclusions: These results provide the basic properties of mouse megakryocyte P2X1 receptors and would be helpful to examine the P2 receptor signaling in platelets and megakaryocytes.
言語 en
内容記述
内容記述タイプ Other
内容記述 http://www.elsevier.com/wps/find/journaldescription.cws_home/369/description
言語 en
書誌情報 en : Thrombosis research

巻 119, 号 3, p. 343-353, 発行日 2007
出版者
出版者 Elsevier
言語 en
ISSN
収録物識別子タイプ ISSN
収録物識別子 00493848
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA00863148
著者版フラグ
出版タイプ AM
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Cite as

池田, 正浩, 2007, Characterization of functional P2X1 receptors in mouse megakaryocytes: Elsevier, 343–353 p.

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