{"created":"2023-05-15T09:57:05.966095+00:00","id":860,"links":{},"metadata":{"_buckets":{"deposit":"ddc7fa5f-aabf-431f-a0d2-29cec0a37355"},"_deposit":{"created_by":5,"id":"860","owner":"5","owners":[5],"pid":{"revision_id":0,"type":"depid","value":"860"},"status":"published"},"_oai":{"id":"oai:miyazaki-u.repo.nii.ac.jp:00000860","sets":["74","74:26"]},"author_link":["1081"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2007","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"353","bibliographicPageStart":"343","bibliographicVolumeNumber":"119","bibliographic_titles":[{"bibliographic_title":"Thrombosis research","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Introduction: Although accumulating evidence within the past five years strongly supports the importance of platelet P2X1 receptors in hemostasis and thrombosis, P2X1 receptors of platelet and /or its progenitor cell, megakaryocyte, have not been fully characterized. The aim of this study was to electrophysiologically and pharamacologically characterize the functional P2X1 receptors on mouse\nmegakaryocytes.\nMaterials and Methods: The currents in response to nucleotides were examined using the patch-clamp whole-cell recording.\nResults: The agonist profile of megakaryocyte P2X1 receptors was ATP >α,β-methyleneATP > β,γ-methyleneATP. The P2X1 receptors exhibited substantial monovalent as well as divalent cation permeability and the ratios of Na+ to Cs+ and Ca2+ to Cs+ permeability were 1 and 2.5, respectively.\nP2X receptor antagonists except suramin significantly inhibited the P2X1 responses with an IC50 values of 0.4 μM for pyridoxal-phosphate-6-azophenyl-2',4'-disulphonate （PPADS）, 0.3 μM for 2',3'-O-（2,4,6-trinitophenyl）- adenosine 5'-triphosphate （TNP-ATP）, 20 μM for reactive blue 2 （RB2）,\nor 160 μM for 8,8'-（carbonylbis（imino-3,1-phenylene carbonylimino）bis（1,3,5-naphthalenetrisulfonic acid） （NF023）, respectively. Suramin had no significant effect on the P2X1 responses. In rat\nmegakaryocytes, suramin similarly had no significant effect on the P2X1 responses, but abolished the P2Y receptor-mediated responses, indicating that the suramin was active under present experimental\ncondition.\nConclusions: These results provide the basic properties of mouse megakryocyte P2X1 receptors and would be helpful to examine the P2 receptor signaling in platelets and megakaryocytes.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_description_6":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"http://www.elsevier.com/wps/find/journaldescription.cws_home/369/description","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier","subitem_publisher_language":"en"}]},"item_10001_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00863148","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00493848","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"池田, 正浩","creatorNameLang":"ja"},{"creatorName":"イケダ, マサヒロ","creatorNameLang":"ja-Kana"},{"creatorName":"Ikeda, Masahiro","creatorNameLang":"en"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-06-21"}],"displaytype":"detail","filename":"TP-2007-Ikeda.pdf","filesize":[{"value":"695.0 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"TP-2007-Ikeda.pdf","url":"https://miyazaki-u.repo.nii.ac.jp/record/860/files/TP-2007-Ikeda.pdf"},"version_id":"01582af0-8175-4fd8-a157-f633a346be39"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"megakaryocytes, platelets, ATP, P2X1, patch-clamp","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Characterization of functional P2X1 receptors in mouse megakaryocytes","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Characterization of functional P2X1 receptors in mouse megakaryocytes","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"5","path":["74","26"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2008-01-29"},"publish_date":"2008-01-29","publish_status":"0","recid":"860","relation_version_is_last":true,"title":["Characterization of functional P2X1 receptors in mouse megakaryocytes"],"weko_creator_id":"5","weko_shared_id":2},"updated":"2023-07-29T13:01:19.482510+00:00"}