@article{oai:miyazaki-u.repo.nii.ac.jp:00000860,
 author = {池田, 正浩 and Ikeda, Masahiro},
 issue = {3},
 journal = {Thrombosis research},
 month = {},
 note = {Introduction: Although accumulating evidence within the past five years strongly supports the importance of platelet P2X1 receptors in hemostasis and thrombosis, P2X1 receptors of platelet and /or its progenitor cell, megakaryocyte, have not been fully characterized. The aim of this study was to electrophysiologically and pharamacologically characterize the functional P2X1 receptors on mouse
megakaryocytes.
Materials and Methods: The currents in response to nucleotides were examined using the patch-clamp whole-cell recording.
Results: The agonist profile of megakaryocyte P2X1 receptors was ATP >α,β-methyleneATP > β,γ-methyleneATP. The P2X1 receptors exhibited substantial monovalent as well as divalent cation permeability and the ratios of Na+ to Cs+ and Ca2+ to Cs+ permeability were 1 and 2.5, respectively.
P2X receptor antagonists except suramin significantly inhibited the P2X1 responses with an IC50 values of 0.4 μM for pyridoxal-phosphate-6-azophenyl-2',4'-disulphonate （PPADS）, 0.3 μM for 2',3'-O-（2,4,6-trinitophenyl）- adenosine 5'-triphosphate （TNP-ATP）, 20 μM for reactive blue 2 （RB2）,
or 160 μM for 8,8'-（carbonylbis（imino-3,1-phenylene carbonylimino）bis（1,3,5-naphthalenetrisulfonic acid） （NF023）, respectively. Suramin had no significant effect on the P2X1 responses. In rat
megakaryocytes, suramin similarly had no significant effect on the P2X1 responses, but abolished the P2Y receptor-mediated responses, indicating that the suramin was active under present experimental
condition.
Conclusions: These results provide the basic properties of mouse megakryocyte P2X1 receptors and would be helpful to examine the P2 receptor signaling in platelets and megakaryocytes., http://www.elsevier.com/wps/find/journaldescription.cws_home/369/description},
 pages = {343--353},
 title = {Characterization of functional P2X1 receptors in mouse megakaryocytes},
 volume = {119},
 year = {2007},
 yomi = {イケダ, マサヒロ}
}