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Molecular Cloning and Recombinant Expression of Tiger Shrimp Penaeus monodon Penaeidin
http://hdl.handle.net/10458/1099
http://hdl.handle.net/10458/10991a29d27e-c99c-4707-8390-23ff8beb970c
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
FP 39(1) 15-23 2004.pdf (4.5 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2008-01-16 | |||||
| タイトル | ||||||
| タイトル | Molecular Cloning and Recombinant Expression of Tiger Shrimp Penaeus monodon Penaeidin | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | penaeidin, antimicrobial peptide, Penaeus monodon, shrimp hemocyte, insectbaculovirus expression system, antimicrobial activity | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| 著者 |
Ho, Shih-Hu
× Ho, Shih-Hu× Chao, Yu-Chan× Tsao, Hsiao-Wei× 酒井, 正博× Chou, Hong-Nong× Song, Yen-Ling |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | A penaeidin cDNA sequence of tiger shrimp Penaeus monodon was obtained. In the sequence, an open reading frame that coded for a peptide composed of 74 amino acids was found. A cleavage site of secretory signal peptide was predicted between amino acids 19 and 20. The calculated molecular mass of mature penaeidin was about 6.1 kDa and the estimated pl of this peptide was 9.1. Northern blot analysis indicated that the penaeidin was mainly synthesized in the hemocytes. Clustering analysis of penaeidin sequences from P. monodon, P. vannamei, P. setiferus, P. japonicus and P. chinensis was performed. The recombinant penaeidin was expressed using insect-baculovirus expression system. The recombinant penaeidin showed antimicrobial activity against a bacterium Aerococcus viridans, but not Vibrio alginolyticus, Vibrio harveyi or a yeast Debaryomyces hansenii. In addition, it delayed spore germination and growth of a filamentous fungus Neurospora crassa. |
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| 言語 | en | |||||
| 書誌情報 |
ja : 魚病研究 en : Fish pathology 巻 39, 号 1, p. 15-23, 発行日 2004-03 |
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| 出版者 | ||||||
| 出版者 | 日本魚病学会 | |||||
| 言語 | ja | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0388788X | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AN00063165 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
