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Effects of Tyrosine Kinase Inhibitor on the Motility and ATP Concentrations of Fowl Spermatozoa
http://hdl.handle.net/10458/734
http://hdl.handle.net/10458/7347de4bf75-b259-4cd6-af95-1472aa4dfb8b
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
ashizawa62.pdf (1.5 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2007-10-11 | |||||
| タイトル | ||||||
| タイトル | Effects of Tyrosine Kinase Inhibitor on the Motility and ATP Concentrations of Fowl Spermatozoa | |||||
| 言語 | en | |||||
| タイトル | ||||||
| タイトル | Tyrosine kinase and fowl sperm motility | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | ja | |||||
| 主題Scheme | Other | |||||
| キーワード | 精子 | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | sperm, Spermatozoa, Protein phosphorylation / dephosphorylation, Tyrosine kinase, Motility | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| 著者 |
芦沢, 幸二
× 芦沢, 幸二× Higashio, Mari× Tsuzuki, Yasuhiro |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The possible role of tyrosine kinase in the regulation of fowl sperm motility was investigated by using a stable analogue of erbstatin, methyl 2,5-dihydroxycinnamate （2,5-MeC）, a specific inhibitor of tyrosine kinase. This inhibited the motility of intact spermatozoa at 30°C in a dose- dependent manner. In contrast, the motility of demembranated spermatozoa was not inhibited by the same concentrations of 2,5-MeC. At 40°C, both intact and demembranated spermatozoa were almost immotile with or without 2,5-MeC. Additionally, intact spermatozoa, stimulated by the addition of Ca2+ or calyculin A, a specific inhibitor of protein phosphatases, lost their motility with the subsequent addition of 2,5-MeC at 40°C. However, unlike the motility, the ATP concentrations of spermatozoa were maintained in about 30-35 nmol ATP/10（9） cells during these incubation periods. The activity of tyrosine kinase of spermatozoa at 30°C, estimated by measuring the phosphorylation of a synthetic peptide substrate, RR-SRC, was 0.17 pmol/min/mg protein. This activity was lower than those of fowl testes and chick brain, but higher than that of chick liver. These results suggest that tyrosine kinase activity, which is not retained in the axoneme and/or accessory cytoskeletal components, may be involved in the maintenance of flagellar movement of fowl spermatozoa at 30°C. | |||||
| 言語 | en | |||||
| 書誌情報 |
en : Molecular reproduction and development 巻 49, 号 1, p. 196-202, 発行日 1998 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1040452X | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA10735246 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
