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The Addition of Mitogen-Activated Protein (MAP) Kinase and p34(cdc2) Kinase Substrate Peptides Inhibits the Flagellar Motility of Demembranated Fowl Spermatozoa
http://hdl.handle.net/10458/721
http://hdl.handle.net/10458/721ca7b0b85-95ba-48e6-b433-beca1fc0ef7d
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
ashizawa61.pdf (1.2 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2007-10-10 | |||||
| タイトル | ||||||
| タイトル | The Addition of Mitogen-Activated Protein (MAP) Kinase and p34(cdc2) Kinase Substrate Peptides Inhibits the Flagellar Motility of Demembranated Fowl Spermatozoa | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | ja | |||||
| 主題Scheme | Other | |||||
| キーワード | 精子 | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | sperm | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| 著者 |
芦沢, 幸二
× 芦沢, 幸二× Hashimoto, Kei× Higashio, Mari× Tsuzuki, Yasuhiro |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The motility of demembranated fowl spermatozoa was vigorous at 30°C, but decreased markedly following the addition of mitogen-activated protein （MAP） kinase or p34（cdc2） kinase substrate peptide. Dephosphorylation of approximately 116, 86 and 79-kDa proteins of demembranated spermatozoa was observed after the addition of MAP kinase or p34（cdc2） kinase substrate peptide. The activities of MAP kinase and histone H1 kinase of spermatozoa, estimated by measuring the phosphorylation of myelin basic protein and histone H1 as substrates, were 1.22 and 0.29 pmol/min/mg protein, respectively. Both enzymatic activities of spermatozoa were lower than those of chick brain, but higher than those of chick liver. These results suggest that the phosphorylation of axonemal and/or accessory cytoskeletal proteins mediated by MAP kinase and p34（cdc2） kinase may be involved in the regulation of flagellar movement of fowl spermatozoa. | |||||
| 言語 | en | |||||
| 書誌情報 |
en : Biochemical and biophysical research communications 巻 240, 号 1, p. 116-121, 発行日 1997 |
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| 出版者 | ||||||
| 出版者 | Elsevier | |||||
| 言語 | en | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0006291X | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA00564395 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
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芦沢, 幸二, Hashimoto, Kei, Higashio, Mari, Tsuzuki, Yasuhiro, 1997, The Addition of Mitogen-Activated Protein (MAP) Kinase and p34(cdc2) Kinase Substrate Peptides Inhibits the Flagellar Motility of Demembranated Fowl Spermatozoa: Elsevier, 116–121 p.
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