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Role of E-cadherin in the induction of apoptosis of HPV16-positive CaSki cervical cancer cells during multicellular tumor spheroid formation
http://hdl.handle.net/10458/2877
http://hdl.handle.net/10458/287749bf2941-4fbb-4639-a34d-90882e7b03d3
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
2877-.pdf (417.2 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2010-05-26 | |||||
| タイトル | ||||||
| タイトル | Role of E-cadherin in the induction of apoptosis of HPV16-positive CaSki cervical cancer cells during multicellular tumor spheroid formation | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | Apoptosis, E-cadherin, Spheroid, Cervical cancer, CaSki cell, EGF | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| 著者 |
Haga, Takeshi
× Haga, Takeshi× Uchide, Noboru× Tugizov, Sharof× Palefsky, Joel M. |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Multicellular tumor spheroids （MCTS） are three dimensional cell culture systems induced by suspension culture. MCTS are widely used in cancer research because of their similarity to solid tumors. CaSki cells are derived from a metastatic cervical cancer containing human papillomavirus 16 （HPV16）. Cell death of CaSki cells in MCTS has been previously reported, and our model is used to better characterize the mechanisms of cell death of HPV16-positive keratinocytes. In this study, we found that apoptosis of CaSki cells was induced by suspension culture along with the formation of MCTS after 24 hours of incubation. In suspended CaSki cells, monoclonal antibodies blocking E-cadherin function inhibited MCTS formation and suppressed suspension-induced apoptosis in a dose-dependent manner. Western blot for E-cadherin detected upregulation of the authentic 120 kDa band from MCTS of CaSki cells as well as a shorter 100 kDa band. Addition of EGF, whose receptor is known to form a complex with E-cadherin, abrogated apoptosis of suspended CaSki cells in a dose-dependent manner. These findings suggest that E-cadherin-dependent cell-cell contact, directly or indirectly, mediates the signal to undergo apoptosis of CaSki cells during MCTS formation, and thus provides new information on the role of E-cadherin in cervical cancer cell apoptosis. | |||||
| 言語 | en | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | The original publication is available at www.springerlink.com | |||||
| 言語 | en | |||||
| 書誌情報 |
en : Apoptosis en : Apoptosis : an international journal on programmed cell death 巻 13, 号 1, p. 97-108, 発行日 2008-01 |
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| 出版者 | ||||||
| 出版者 | Springer Netherlands | |||||
| 言語 | en | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 13608185 | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1573675X | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA11148723 | |||||
| PubMed番号 | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 17906929 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
