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Generation of Nonaploid Persimmons (Diospyros kaki Thunb.) by Embryo Culture of Imperfect Seeds Derived from a Cross between 'Fuyu' and 'Taishuu'
http://hdl.handle.net/10458/2767
http://hdl.handle.net/10458/2767fa618e1c-cb85-4ddd-85bc-0029b568cad5
名前 / ファイル | ライセンス | アクション |
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2010-03-29 | |||||
タイトル | ||||||
タイトル | Generation of Nonaploid Persimmons (Diospyros kaki Thunb.) by Embryo Culture of Imperfect Seeds Derived from a Cross between 'Fuyu' and 'Taishuu' | |||||
言語 | en | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
キーワード | embryo culture, meiosis, pollination-constant non-astringent (PCNA), sexual polyploidy, unreduced gamete | |||||
資源タイプ | ||||||
資源タイプ | journal article | |||||
著者 |
Chijiwa, Hiroyuki
× Chijiwa, Hiroyuki× Kuwahara, Minoru× Hirakawa, Nobuyuki× 鉄村, 琢哉 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | We obtained nonaploid plants by embryo culture of imperfect seeds derived from a cross between 'Fuyu' and 'Taishuu', both of which are commercially important persimmon cultivars. Furthermore, we aimed to clarify the origin of nonaploid plants derived from imperfect seeds. Of 1078 seeds, 68 were imperfect; this accounted for 6.3% of the total number. Ten of the 68 seeds germinated into seedlings, and two produced abnormal plantlets without meristems at both the shoot apex and root tip. The remaining eight produced normal-appearing plantlets with normal growth. Two seedlings were recovered from the abnormal plantlets via callus, and grew vigorously in the greenhouse. Cytogenetic analysis confirmed that these two seedlings were nonaploids. Parental analysis using four simple sequence repeats (SSR) markers, D.CT-13, 24, 61 and 179, showed that each nonaploid seedling had alleles originating from the parents, indicating that they were generated by syngamy. The two nonaploid seedlings had alleles of 222 bp at D.CT-61, which is peculiar to the pollen parent, 'Taishuu', whereas they did not have an allele of 136 or 140 bp at D.CT-179, which is peculiar to the seed parent, 'Fuyu'. These results suggest that they might be derived from fertilization of a reduced female gamete with an unreduced male gamete. | |||||
言語 | en | |||||
書誌情報 |
en : Journal of the Japanese Society for Horticultural Science en : Engei Gakkai zasshi 巻 77, 号 4, p. 358-363, 発行日 2008-10 |
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出版者 | ||||||
出版者 | Japanese Society for Horticultural Science | |||||
言語 | en | |||||
出版者 | ||||||
出版者 | 園芸学会 | |||||
言語 | ja | |||||
出版者 | ||||||
出版者 | The Japanese Society for Horticultural Science | |||||
言語 | en | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 18823351 | |||||
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収録物識別子タイプ | NCID | |||||
収録物識別子 | AA12177046 | |||||
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出版タイプ | VoR |