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Characterization of human telomere RNA G-quadruplex structures in vitro and in living cells using 19F NMR spectroscopy
http://hdl.handle.net/10458/6670
http://hdl.handle.net/10458/6670208652ea-6c90-4868-8c40-449ed7bed730
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youshi.pdf (5.3 MB)
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shinsakekka.pdf (499.0 kB)
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honbun.pdf (3.1 MB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2020-06-21 | |||||
| タイトル | ||||||
| タイトル | Characterization of human telomere RNA G-quadruplex structures in vitro and in living cells using 19F NMR spectroscopy | |||||
| タイトル | ||||||
| タイトル | 19F NMR分光法を用いた的vitroおよび生きた細胞におけるヒトテロメアRNA G-quadruplex構造の解析 | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||
| 資源タイプ | doctoral thesis | |||||
| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| 著者 |
鮑, 宏亮
× 鮑, 宏亮× Hong, Liang BAO |
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| 公開者 | ||||||
| 出版者 | Elsevier | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Human telomeric RNA has been identified as a key component of the telomere machinery. Recently, the growing evidence suggests that the telomeric RNA forms G-quadruplex structures to play an important role in telomere protection and regulation. In the present studies, we developed a 19F NMR spectroscopy method to investigate the telomeric RNA G-quadruplex structures in vitro and in living cells. We demonstrated that the simplicity and sensitivity of 19F NMR approach can be used to directly observe the dimeric and two-subunits stacked Gquadruplexes in vitro and in living cells and quantitatively characterize the thermodynamic properties of the G-quadruplexes. By employing the 19F NMR in living cell experiment, we confirmed for the first time that the higher-order G-quadruplex exists in cells. We further demonstrated that telomere RNA G-quadruplexes are converted to the higher-order Gquadruplex under molecular crowding condition, a cell-like environment. We also show that the higherorder G-quadruplex has high thermal stability in crowded solutions. The finding provides new insight into the structural behavior of telomere RNA G-quadruplex in living cells. These results open new avenues for the investigation of G-quadruplex structures in vitro and in living cells. | |||||
| 内容記述 | ||||||
| 以下に掲載: Nuc/eic Acids Research, 2017,Volume 45, No.9, pages 5501-5511 | ||||||
| 学位名 | ||||||
| 学位名 | 博士(医学) | |||||
| 学位授与機関 | ||||||
| 学位授与機関識別子Scheme | kakenhi | |||||
| 学位授与機関識別子 | 17601 | |||||
| 学位授与機関名 | 宮崎大学 | |||||
| 学位授与年月日 | ||||||
| 学位授与年月日 | 2019-03-22 | |||||
| 学位授与番号 | ||||||
| 学位授与番号 | 甲第91号 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
