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Generation of Pax5-deficient DT40 mutant cells, Pax5(-), and protein and mRNA levels of IgM H- and L-chains artificially and excessively accumulated in Pax5(-) DT40 mutants are rapidly and dramatically reduced through various generations during continuous cultivation
http://hdl.handle.net/10458/6503
http://hdl.handle.net/10458/65036d6cadb5-54de-4911-8a9f-cb934715d1a1
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| Item type | その他 / Others(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2020-06-21 | |||||
| タイトル | ||||||
| タイトル | Generation of Pax5-deficient DT40 mutant cells, Pax5(-), and protein and mRNA levels of IgM H- and L-chains artificially and excessively accumulated in Pax5(-) DT40 mutants are rapidly and dramatically reduced through various generations during continuous cultivation | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Pax5-deficient DT40 mutants, continuous cultivation, decreases in IgM H/L chain protein/mRNA levels, changes in mRNA levels of transcription factors, gene targeting techniques | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_1843 | |||||
| 資源タイプ | other | |||||
| 著者 |
中山, 雅美
× 中山, 雅美× 中山, 建男 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | We generated Pax5-deficient DT40 mutant cells, Pax5（-）, devoid of the Pax5 gene existing on Z sex chromosome that is monosomy in chickens and analyzed characteristics of Pax5（-） by Western blotting, microscopy, electron microscopy, immuno-electron microscopy and reverse transcription-polymerase chain reaction （RT-PCR）. Protein levels of IgM H- and L-chains in Pax5（-） were drastically increased at the early stage of cultivation and thereafter dramatically decreased during cultivation and finally at the later stage reached comparable levels in DT40 cells. Whole and secreted forms of IgM H-chain mRNA in Pax5（-） were dramatically elevated at the first stage and thereafter rapidly reduced until the later stage to comparable levels in DT40 cells. The membrane-bound form of IgM H-chain mRNA and IgM L-chain mRNA were considerably increased at the first stage and thereafter slowly decreased during cultivation. In addition, PCAF and HDAC9 mRNA levels were gradually elevated during cultivation, and the HDAC7 mRNA level moderately changed. Aiolos and OBF1 mRNA levels were gradually reduced from the first to later stages, whereas Ikaros and E2A mRNA levels were drastically elevated at the first stage and gradually decreased until the later stage. The EBF1 mRNA level was completely decreased at the first stage and remained unchanged during cultivation. The PU.1 mRNA level was remarkably reduced at the first stage and gradually elevated until the later stage. These results that Pax5 dramatically down-regulates gene expressions of IgM H- and L-chains, together with our previous findings, revealed not only that gene expressions of the two immunoglobulin proteins are indirectly regulated by HDAC2 through opposite regulations of gene expressions of Pax5, Aiolos, EBF1, Ikaros and E2A in DT40 cells, but also that the decreases in the elevated immunoglobulin protein and mRNA levels during cultivation are more rapid in Pax5（-） than in HDAC2（-/-）. In addition, the influences of the Pax5-deficiency on several cellular characteristics should be certainly different from those of the HDAC2-deficiency. |
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| 言語 | en | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | The original edition of the revision is available from following URL: http://opac2.lib.miyazaki-u.ac.jp/webopac/TC10145176 | |||||
| 言語 | en | |||||
| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
