WEKO3
アイテム
There is a newer version of this record available.
{"_buckets": {"deposit": "006071f6-ab75-4366-b41f-a65a65d6660f"}, "_deposit": {"created_by": 9, "id": "5336.2", "owner": "9", "owners": [9], "pid": {"revision_id": 0, "type": "depid", "value": "5336.2"}, "status": "published"}, "_oai": {"id": "oai:miyazaki-u.repo.nii.ac.jp:00005336.2", "sets": ["76", "63"]}, "author_link": ["28840", "28841", "9885", "28843", "28844", "28845", "28846", "28847", "9886"], "item_10006_date_granted_11": {"attribute_name": "学位授与年月日", "attribute_value_mlt": [{"subitem_dategranted": "2017-03-24"}]}, "item_10006_degree_grantor_9": {"attribute_name": "学位授与機関", "attribute_value_mlt": [{"subitem_degreegrantor": [{"subitem_degreegrantor_language": "ja", "subitem_degreegrantor_name": "宮崎大学"}], "subitem_degreegrantor_identifier": [{"subitem_degreegrantor_identifier_name": "17601", "subitem_degreegrantor_identifier_scheme": "kakenhi"}]}]}, "item_10006_degree_name_8": {"attribute_name": "学位名", "attribute_value_mlt": [{"subitem_degreename": "博士(医学)", "subitem_degreename_language": "ja"}]}, "item_10006_description_7": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Cancer-associated fibroblasts (CAFs) are known to contribute to cancer progression. We have reported that cell surface expression of hepatocyte growth factor activator inhibitor 1 (HAI-1) is decreased in invasive oral squamous cell carcinoma (OSCC) cells. This study examined if HAI-1-insufficiency contributes to CAF recruitment in OSCC. Serum-free conditioned medium (SFCM) from a human OSCC line (SAS) stimulated the migration of 3 human fibroblast cell lines, NB1RGB, MRC5 and KD. SFCM from HAI-1-knockdown SAS showed an additive effect on the migration of NB1RGB and MRC5, but not KD. SAS SFCM induced protease-activated receptor-2 (PAR-2) expression in NB1RGB and MRC5, but not in KD, and a PAR-2 antagonist blocked the stimulatory effect of HAI-1 knockdown on migration of the PAR-2 expressing cell lines. Moreover, HAI-1-deficient SFCM showed additive stimulatory effects on the migration of wild-type but not PAR-2-deficient mouse fibroblasts. Therefore, the enhanced migration induced by HAI-1-insufficiency was mediated by PAR-2 activation in fibroblasts. This activation resulted from the deregulation of the activity of matriptase, a PAR-2 agonist protease. HAI-1 may thus prevent CAF recruitment to OSCC by controlling matriptase activity. When HAI-1 expression is reduced on OSCC, matriptase may contribute to CAF accumulation by paracrine activation of fibroblast PAR-2. Immunohistochemical analysis of resected OSCC revealed increased PAR2-positive CAFs in 35% (33/95) of the cases studied. The increased PAR-2 positive CAFs tended to correlate with infiltrative histology of the invasion front and shorter disease-free survival of the patients.", "subitem_description_language": "en", "subitem_description_type": "Abstract"}]}, "item_10006_dissertation_number_12": {"attribute_name": "学位授与番号", "attribute_value_mlt": [{"subitem_dissertationnumber": "甲第50号"}]}, "item_10006_publisher_5": {"attribute_name": "公開者", "attribute_value_mlt": [{"subitem_publisher": "Wiley", "subitem_publisher_language": "en"}]}, "item_10006_relation_15": {"attribute_name": "関連サイト", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_language": "en", "subitem_relation_name_text": "http://hdl.handle.net/10458/6184"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "http://hdl.handle.net/10458/6184", "subitem_relation_type_select": "HDL"}}]}, "item_10006_text_24": {"attribute_name": "BIBID", "attribute_value_mlt": [{"subitem_text_value": "TC10164453"}]}, "item_10006_textarea_22": {"attribute_name": "内容記述", "attribute_value_mlt": [{"subitem_textarea_value": "以下に掲載:International Journal of Cancer .2017, Volume140, Issue 1, pp 130-141, doi:http://dx.doi.org/10.1002/ijc.30426"}, {"subitem_textarea_language": "ja"}]}, "item_10006_version_type_18": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "open access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_abf2"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "金丸, 愛", "creatorNameLang": "ja"}, {"creatorName": "カネマル, アイ", "creatorNameLang": "ja-Kana"}], "nameIdentifiers": [{"nameIdentifier": "28840", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kanemaru, Ai", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "28841", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yamamoto, Koji", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "9885", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kawaguchi, Makiko", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "28843", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Fukushima, Tsuyoshi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "28844", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Lin, Chen-Yong", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "28845", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Michael, D. Johnson", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "28846", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Eric, Camerer", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "28847", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kataoka, Hiroaki", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "9886", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "10214321", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=10214321"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-06-21"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "2017_kanemaru.pdf", "filesize": [{"value": "11.6 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 11600000.0, "url": {"label": "学位論文要旨", "url": "https://miyazaki-u.repo.nii.ac.jp/record/5336/files/2017_kanemaru.pdf"}, "version_id": "4dd8c931-b58f-4b44-ba4e-97704c131efd"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-06-21"}], "displaytype": "detail", "download_preview_message": "", "file_order": 1, "filename": "youshi.pdf", "filesize": [{"value": "206.5 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 206500.0, "url": {"label": "学位論文審査結果の要旨", "url": "https://miyazaki-u.repo.nii.ac.jp/record/5336/files/youshi.pdf"}, "version_id": "4c07d84b-2d84-401d-bdca-4d0a5a6aa043"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-06-21"}], "displaytype": "detail", "download_preview_message": "", "file_order": 2, "filename": "shinsakekka.pdf", "filesize": [{"value": "52.2 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 52200.0, "url": {"label": "本文", "url": "https://miyazaki-u.repo.nii.ac.jp/record/5336/files/shinsakekka.pdf"}, "version_id": "54ac36cd-35e2-4e21-951e-9f7bd23d57c6"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "doctoral thesis", "resourceuri": "http://purl.org/coar/resource_type/c_db06"}]}, "item_title": "Deregulated matriptase activity in oral squamous cell carcinoma promotes the infiltration of cancer-associated fibroblasts by paracrine activation of protease-activated receptor 2", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Deregulated matriptase activity in oral squamous cell carcinoma promotes the infiltration of cancer-associated fibroblasts by paracrine activation of protease-activated receptor 2", "subitem_title_language": "en"}]}, "item_type_id": "10006", "owner": "9", "path": ["76", "63"], "permalink_uri": "http://hdl.handle.net/10458/6185", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2020-06-21"}, "publish_date": "2020-06-21", "publish_status": "0", "recid": "5336.2", "relation": {}, "relation_version_is_last": true, "title": ["Deregulated matriptase activity in oral squamous cell carcinoma promotes the infiltration of cancer-associated fibroblasts by paracrine activation of protease-activated receptor 2"], "weko_shared_id": 2}
Deregulated matriptase activity in oral squamous cell carcinoma promotes the infiltration of cancer-associated fibroblasts by paracrine activation of protease-activated receptor 2
http://hdl.handle.net/10458/6185
http://hdl.handle.net/10458/6185c27ae1c4-8d53-41aa-8bca-fe9873dc628a
名前 / ファイル | ライセンス | アクション |
---|---|---|
学位論文要旨 (11.6 MB)
|
|
|
学位論文審査結果の要旨 (206.5 kB)
|
|
|
本文 (52.2 kB)
|
|
Item type | 学位論文 / Thesis or Dissertation(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2020-06-21 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Deregulated matriptase activity in oral squamous cell carcinoma promotes the infiltration of cancer-associated fibroblasts by paracrine activation of protease-activated receptor 2 | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||
資源タイプ | doctoral thesis | |||||
アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
著者 |
金丸, 愛
× 金丸, 愛× Kanemaru, Ai× Yamamoto, Koji× Kawaguchi, Makiko× Fukushima, Tsuyoshi× Lin, Chen-Yong× Michael, D. Johnson× Eric, Camerer× Kataoka, Hiroaki |
|||||
公開者 | ||||||
言語 | en | |||||
出版者 | Wiley | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Cancer-associated fibroblasts (CAFs) are known to contribute to cancer progression. We have reported that cell surface expression of hepatocyte growth factor activator inhibitor 1 (HAI-1) is decreased in invasive oral squamous cell carcinoma (OSCC) cells. This study examined if HAI-1-insufficiency contributes to CAF recruitment in OSCC. Serum-free conditioned medium (SFCM) from a human OSCC line (SAS) stimulated the migration of 3 human fibroblast cell lines, NB1RGB, MRC5 and KD. SFCM from HAI-1-knockdown SAS showed an additive effect on the migration of NB1RGB and MRC5, but not KD. SAS SFCM induced protease-activated receptor-2 (PAR-2) expression in NB1RGB and MRC5, but not in KD, and a PAR-2 antagonist blocked the stimulatory effect of HAI-1 knockdown on migration of the PAR-2 expressing cell lines. Moreover, HAI-1-deficient SFCM showed additive stimulatory effects on the migration of wild-type but not PAR-2-deficient mouse fibroblasts. Therefore, the enhanced migration induced by HAI-1-insufficiency was mediated by PAR-2 activation in fibroblasts. This activation resulted from the deregulation of the activity of matriptase, a PAR-2 agonist protease. HAI-1 may thus prevent CAF recruitment to OSCC by controlling matriptase activity. When HAI-1 expression is reduced on OSCC, matriptase may contribute to CAF accumulation by paracrine activation of fibroblast PAR-2. Immunohistochemical analysis of resected OSCC revealed increased PAR2-positive CAFs in 35% (33/95) of the cases studied. The increased PAR-2 positive CAFs tended to correlate with infiltrative histology of the invasion front and shorter disease-free survival of the patients. | |||||
言語 | en | |||||
内容記述 | ||||||
以下に掲載:International Journal of Cancer .2017, Volume140, Issue 1, pp 130-141, doi:http://dx.doi.org/10.1002/ijc.30426 | ||||||
内容記述 | ||||||
ja | ||||||
学位名 | ||||||
言語 | ja | |||||
学位名 | 博士(医学) | |||||
学位授与機関 | ||||||
学位授与機関識別子Scheme | kakenhi | |||||
学位授与機関識別子 | 17601 | |||||
言語 | ja | |||||
学位授与機関名 | 宮崎大学 | |||||
学位授与年月日 | ||||||
学位授与年月日 | 2017-03-24 | |||||
学位授与番号 | ||||||
学位授与番号 | 甲第50号 | |||||
関連サイト | ||||||
識別子タイプ | HDL | |||||
関連識別子 | http://hdl.handle.net/10458/6184 | |||||
言語 | en | |||||
関連名称 | http://hdl.handle.net/10458/6184 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |