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Protein phosphatase-type 2B (PP2B) involved in the regulation of the acrosome reaction, but not in the temperature-dependent flagellar movement of fowl spermatozoa
http://hdl.handle.net/10458/722
http://hdl.handle.net/10458/722ac08d365-3ea6-4fe4-b196-d02d831aec98
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
ashizawa78.pdf (943.0 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2007-10-10 | |||||
| タイトル | ||||||
| タイトル | Protein phosphatase-type 2B (PP2B) involved in the regulation of the acrosome reaction, but not in the temperature-dependent flagellar movement of fowl spermatozoa | |||||
| 言語 | en | |||||
| タイトル | ||||||
| タイトル | Acrosome reaction and motility of fowl spermatozoa | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | ja | |||||
| 主題Scheme | Other | |||||
| キーワード | 精子 | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | sperm | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| 著者 |
芦沢, 幸二
× 芦沢, 幸二× Wishart, G.J.× Ranasinghe, A.R.A.H.× Katayama, S.× Tsuzuki, Y. |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The motility and acrosomal integrity of fowl spermatozoa in TES/NaCl buffer, with or without homogenized inner perivitelline layers （IPVL） prepared from laid fowl eggs, was almost negligible at 40°C. However, motility became vigorous even at 40°C when 2 mmol CaCl2/l was added, and the acrosome reaction was also stimulated in the presence, but not in the absence, of IPVL. The presence of deltamethrin or fenvalerate, specific inhibitors of protein phosphatase-type 2B （PP2B）, did not permit the restoration of motility at 40°C, but, in the presence of IPVL, these compounds stimulated the acrosome reaction in a dose-dependent manner in the range of 1-1000 nmol（-1）. These results suggest that IPVL is necessary for the activation of the acrosome reaction in fowl spermatozoa and that Ca2+ plays an important role in the stimulation of motility and acrosomal exocytosis. Furthermore, it appears that the intracellular molecular mechanisms for the regulation of the acrosome reaction of fowl spermatozoa are different from those for the restoration of motility, i.e., protein dephosphorylation by PP2B in the former but not in the latter case. | |||||
| 言語 | en | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Disclaimer. This is not the definitive version of record of this article. This manuscript has been accepted for publication in Reproduction, but the version presented here has not yet been copy edited, formatted or proofed. Consequently, neither Reproduction, the Society for Reproduction and Fertility nor its publisher, BioScientifica Ltd, accepts responsibility for any errors or omissions it may contain. The definitive version is now freely available at http://dx.doi.org/10.1530/rep.1.00327. 2004 Society for Reproduction and Fertility. | |||||
| 言語 | en | |||||
| 書誌情報 |
en : Reproduction : the official journal of the Society for the Study of Fertility 巻 128, 号 6, p. 783-787, 発行日 2004 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 14701626 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA11501251 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
