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Regulation of flagellar motility of fowl spermatozoa: Evidence for the involvement of intracellular free Ca2+ and calmodulin
http://hdl.handle.net/10458/711
http://hdl.handle.net/10458/7112de93ad3-7b37-4289-b211-e1027743b52b
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
ashizawa48.pdf (1.8 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2007-10-10 | |||||
| タイトル | ||||||
| タイトル | Regulation of flagellar motility of fowl spermatozoa: Evidence for the involvement of intracellular free Ca2+ and calmodulin | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | ja | |||||
| 主題Scheme | Other | |||||
| キーワード | 精子 | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | sperm, spermatozoa, calcium, calmodulin, motility, fowl | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| その他（別言語等）のタイトル | ||||||
| その他のタイトル | Ca2+ and calmodulin regulation in fowl sperm | |||||
| 言語 | en | |||||
| 著者 |
芦沢, 幸二
× 芦沢, 幸二× Tomonaga, H.× 續木, 靖浩 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The possible role of intracellular free Ca2+ and calmodulin in the regulation fowl sperm motility was investigated by using an intracellular Ca2+ chelator, 1,2-bis (2-aminophenoxy) ethane-N,N,N',N'-tetraacetic acid, tetraacetoxymethyl ester (BAPTA/AM) and calmodulin antagonists such as N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide hydrochloride (W-7), N-(6-aminohexyl)-1-naphthalenesulfonamide hydrochloride (W-5) and trifluoperazine. Intact fowl spermatozoa maintained vigorous movement in a Ca2+-free medium at 30°C. In contrast, the motility of spermatozoa loaded with BAPTA/AM was negligible at 30°C, but could be instantly restored by the addition of 2 mmol CaCl2 l-1. At this time, the intracellular free Ca2+ concentrations increased from 0 to about 100 nmol l-1, measured by the fluorescent Ca2+ indicator fura-2. At 40°C, neither control nor BAPTA/AM-treated spermatozoa were motile, but the motility of both spermatozoa was restored by the subsequent addition of 2 mmol CaCl2 l-1. Even in the presence of 2 mmol CaCl2 l-1, the addition of W-7 and trifluoperazine inhibited the motility of intact spermatozoa at 30°C and 40°C, and induced a concomitant decrease in the rate of oxygen consumption and ATP concentration, suggesting that energy depletion might be involved in the inhibition of motility. In contrast, the motility of demembranated spermatozoa was not inhibited by the addition of W-7 and trifluoperazine at 30°C. The addition of W-5, a weaker antagonist, did not appreciably affect the motility of either intact or demembranated spermatozoa. These results suggest that intracellular free Ca2+ is indispensable for the maintenance of fowl sperm motility. and calmodulin which is in the cytoplasm and/or mitochondria, but not retained in the axoneme, is a prominent candidate for the signal transducer in Ca2+-stimulated motility. | |||||
| 言語 | en | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Disclaimer. This is not the definitive version of record of this article. This manuscript has been accepted for publication in Reproduction, but the version presented here has not yet been copy edited, formatted or proofed. Consequently, neither Reproduction, the Society for Reproduction and Fertility nor its publisher, BioScientifica Ltd, accepts responsibility for any errors or omissions it may contain. The definitive version is now freely available at [insert DOI link]. 1994 Society for Reproduction and Fertility. | |||||
| 言語 | en | |||||
| 書誌情報 |
en : Journal of reproduction and fertility 巻 101, 号 2, p. 265-272, 発行日 1994 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 00224251 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA00705952 | |||||
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| 出版タイプ | AM | |||||
