WEKO3
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Enhanced detection of Porcine reproductive and respiratory syndrome virus in fixed tissues by in situ hybridization following tyramide signal amplification
http://hdl.handle.net/10458/5633
http://hdl.handle.net/10458/5633ea4d3214-f281-4feb-8522-62fb1a5a448e
| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||||||
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| 公開日 | 2020-06-21 | |||||||||||||||||
| タイトル | ||||||||||||||||||
| タイトル | Enhanced detection of Porcine reproductive and respiratory syndrome virus in fixed tissues by in situ hybridization following tyramide signal amplification | |||||||||||||||||
| 言語 | en | |||||||||||||||||
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| 言語 | eng | |||||||||||||||||
| キーワード | ||||||||||||||||||
| 言語 | en | |||||||||||||||||
| キーワード | in situ hybridization, lung, porcine reproductive and respiratory syndrome virus | |||||||||||||||||
| 資源タイプ | ||||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||
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| アクセス権 | metadata only access | |||||||||||||||||
| 著者 |
Nguyen, Thi Trang
× Nguyen, Thi Trang× 平井, 卓哉
WEKO
25970
× Pham, Hong Ngan× Nguyen, Thi Lan× 福家, 直幸
WEKO
34661
× 渡山, 恵子× Yamamoto, Tsukasa× 山口, 良二 |
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| 抄録 | ||||||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||||||
| 内容記述 | This study evaluated the sensitivity of biotinyl-tyramide-based in situ hybridization （TISH） method by comparison with chromogenic in situ hybridization （CISH） and immunohistochemical staining （IHC） methods. This study also determined the effect of fixative and fixation time on the detection of Porcine reproductive and respiratory syndrome virus （PRRSV） in paraffin-embedded tissues. Lung samples were fixed in 4% paraformaldehyde （PFA） or 10% neutral buffered formalin （NBF） for various times before paraffin embedding. Of 30 paraffin-embedded lung samples, fixed for 1 day in 4% PFA or 10% NBF, 18 （60%） were positive for PRRSV by nested reverse transcription polymerase chain reaction （nRT-PCR）. All 18 lung samples （100%） also were positive for PRRSV by TISH, but only 10 of these 18 specimens （56%） were positive for PRRSV by IHC and CISH. We demonstrated that TISH can detect PRRSV RNA in paraffin-embedded tissues after up to 90 days of fixation. PRRSV nucleic acids and antigens were better preserved in 4% PFA than in 10% NBF. Compared with CISH and IHC testing methods, TISH appeared to be more sensitive for the detection of PRRSV in paraffin-embedded tissues. | |||||||||||||||||
| 言語 | en | |||||||||||||||||
| 書誌情報 |
en : Journal of Veterinary Diagnostic Investigation 巻 27, 号 3, p. 326-331, 発行日 2015-05 |
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| 出版者 | SAGE Publications | |||||||||||||||||
| 言語 | en | |||||||||||||||||
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| 出版者 | American Association of Veterinary Laboratory Diagnosticians | |||||||||||||||||
| 言語 | en | |||||||||||||||||
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| 収録物識別子タイプ | ISSN | |||||||||||||||||
| 収録物識別子 | 10406387 | |||||||||||||||||
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| 関連タイプ | isVersionOf | |||||||||||||||||
| 識別子タイプ | DOI | |||||||||||||||||
| 関連識別子 | https://doi.org/10.1177/1040638715579260 | |||||||||||||||||
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| 出版タイプ | NA | |||||||||||||||||
