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Detection of porcine reproductive and respiratory syndrome virus in oral fluid from naturally infected pigs in a breeding herd
http://hdl.handle.net/10458/5632
http://hdl.handle.net/10458/5632d32cc4bb-a073-43ab-89b4-7eb58c8d6573
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2020-06-21 | |||||
| タイトル | ||||||
| タイトル | Detection of porcine reproductive and respiratory syndrome virus in oral fluid from naturally infected pigs in a breeding herd | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| キーワード | acclimatization, localization, oral fluid, porcine reproductive and respiratory syndrome virus, tonsil | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| 著者 |
Nguyen, Thi Trang
× Nguyen, Thi Trang× 平井, 卓哉× Yamamoto, Tsukasa× Matsuda, Mari× Okumura, Naoko× フン ザン, グエン ティ× ラン, グエン ティ× 山口, 良二 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The objectives of the present study were to evaluate the anatomic localization of porcine reproductive and respiratory syndrome virus （PRRSV） in naturally infected pigs and to determine whether oral fluid could be used to detect the virus in infected animals. Two sows, seven 2-month-old grower pigs, and 70 6-month-old gilts were included in this study. PRRSV in sera and oral fluid were identified by nested reverse transcription PCR （nRT-PCR） while lung, tonsil, and tissue associated with oral cavity were subjected to nRT-PCR, immunohistochemistry, and in situ hybridization. In sows, PRRSV was identified in oral fluid and tonsils. PRRSV was also detected in oral fluid, tonsils, salivary glands, oral mucosa, and lungs of all seven grower pigs. However, viremia was observed in only two grower pigs. Double staining revealed that PRRSV was distributed in macrophages within and adjacent to the tonsillar crypt epithelium. In gilts, the North American type PRRSV field strain was detected 3 to 8 weeks after introducing these animals onto the farm. These results confirm previous findings that PRRSV primarily replicates in tonsils and is then shed into oral fluid. Therefore, oral fluid sampling may be effective for the surveillance of PRRSV in breeding herds. | |||||
| 言語 | en | |||||
| 書誌情報 |
Journal of Veterinary Science 巻 15, 号 3, p. 361-367, 発行日 2014-09 |
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| 出版者 | ||||||
| 出版者 | Korean Society of Veterinary Science | |||||
| 言語 | en | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1229845X | |||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.4142/jvs.2014.15.3.361 | |||||
| 権利 | ||||||
| 権利情報 | http://creativecommons.org/licenses/by-nc/3.0/ | |||||
| 著者版フラグ | ||||||
| 出版タイプ | NA | |||||
